Mechanosensitive channel Piezo1 induces cell apoptosis in pancreatic cancer by ultrasound with microbubbles

被引：41

作者：

Song, Yue ^{[1
,2
]}

Chen, Jifan ^{[1
,2
]}

Zhang, Cong ^{[1
,2
]}

Xin, Lei ^{[1
,2
]}

Li, Qunying ^{[1
,2
]}

Liu, Yajing ^{[1
,2
]}

Zhang, Chao ^{[1
,2
]}

Li, Shiyan ^{[3
]}

Huang, Pintong ^{[1
,2
]}

机构：

[1] Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Ultrasound Med, Hangzhou 310009, Peoples R China

[2] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Res Ctr Ultrasound Med & Biomed Engn, Hangzhou 310009, Peoples R China

[3] Zhejiang Univ, Dept Ultrasound Med, Affiliated Sir Run Run Shaw Hosp, Sch Med, Hangzhou 310009, Peoples R China

来源：

ISCIENCE | 2022年 / 25卷 / 02期

基金：

中国国家自然科学基金; 国家重点研发计划; 中国博士后科学基金;

关键词：

INTENSITY PULSED ULTRASOUND; DRUG; CAVITATION; DESTRUCTION; MECHANISM; PROTEINS; DELIVERY;

D O I：

10.1016/j.isci.2022.103733

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ultrasound (US), as a safe and non-invasive tool, has drawn researchers' attention to treat pancreatic ductal adenocarcinoma (PDAC). Piezo1, a mechanosensitive channel, can be activated by various mechanical stimuli. In this study, we tested the expression of Piezo1 in PDAC cell lines and tissues, and cell apoptosis in vitro and in vivo with siRNA, a lentivirus system, and a subcutaneous xenograft tumor-bearing model under the condition of US with microbubbles (MBs). We found that Piezo1 was highly expressed in PDAC cells; it was activated by US with MBs and was closely related to the apoptosis of PDAC cell lines and tumors. This study highlighted the idea of utilizing the high expression of Piezo1 in PDAC and US with MBs to provide a non-invasive strategy for the treatment of PDAC from the aspect of mechanotransduction.

引用

页数：17

共 39 条

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