Unravelling biological macromolecules with cryo-electron microscopy

被引：290
|
作者：
Fernandez-Leiro, Rafael [1 ]
Scheres, Sjors H. W. [1 ]
机构：
[1] MRC Lab Mol Biol, Cambridge Biomed Campus, Cambridge CB2 0QH, England
来源：
NATURE | 2016年 / 537卷 / 7620期
基金：
英国医学研究理事会;
关键词：
CRYO-EM STRUCTURE; BEAM-INDUCED MOTION; U4/U6.U5; TRI-SNRNP; ELECTRON CRYOMICROSCOPY; ANGSTROM RESOLUTION; ATOMIC-STRUCTURE; MOLECULAR ARCHITECTURE; DYNACTIN COMPLEX; TRPV1; STRUCTURES; EF-TU;
D O I：
10.1038/nature19948
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Knowledge of the three-dimensional structures of proteins and other biological macromolecules often aids understanding of how they perform complicated tasks in the cell. Because many such tasks involve the cleavage or formation of chemical bonds, structural characterization at the atomic level is most useful. Developments in the electron microscopy of frozen hydrated samples (cryo-electron microscopy) are providing unprecedented opportunities for the structural characterization of biological macromolecules. This is resulting in a wave of information about processes in the cell that were impossible to characterize with existing techniques in structural biology.
引用
收藏
页码：339 / 346
页数：8
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