A new method for the determination of selected PAHs in coffee brew samples by HPLC with fluorimetric detection and solid-phase extraction

被引：32

作者：

Kayali-Sayadi, MN ^{[1
]}

Rubio-Barroso, S ^{[1
]}

Cuesta-Jimenez, MP ^{[1
]}

Polo-Díez, LM ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Chem, Dept Analyt Chem, E-28040 Madrid, Spain

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1999年 / 22卷 / 04期

关键词：

D O I：

10.1081/JLC-100101686

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid analytical method is proposed for the determination of PAHs (fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[e]pyrene, benzo[a]pyrene, dibenzo[a]anthracene and benzo[g,h,i]perylene) in coffee brew samples. The method is based on solid phase extraction with Sep-Pak Vac tC-18 cartridges and elution with ethyl ether. The extracts were analyzed by RP-HPLC with a Green PAH column and fluorescence detection under an acetonitrile-water mobile phase gradient. Some PAHs were detected in the samples studied and their mean contents were 1.65-2.87 ng/L coffee brew. The relative standard deviations were in the range 2-13% for four replicates.

引用

页码：615 / 627

页数：13

共 22 条

[1] Baird, 1995, ENVIRON CHEM, P215
[2] BELITZ HD, 1991, DEUTSCH LEBENSM RUND, V87, P69
[3] RAPID-DETERMINATION OF BENZO[A]PYRENE IN ROASTED COFFEE AND COFFEE BREW BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
DEKRUIJF, N
SCHOUTEN, T
VANDERSTEGEN, GHD
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1987, 35 (04) : 545 - 549
[4] POLYCYCLIC AROMATIC-HYDROCARBONS IN DUTCH TOTAL DIET SAMPLES (1984-1986)
DEVOS, RH
VANDOKKUM, W
SCHOUTEN, A
DEJONGBERKHOUT, P
[J]. FOOD AND CHEMICAL TOXICOLOGY, 1990, 28 (04) : 263 - 268
[5] DOLAN JW, 1995, LC GC INT, V8, P381
[6] Solid-phase microextraction coupled to gas chromatography: A new method for the analysis of organics in water
Eisert, R
Levsen, K
[J]. JOURNAL OF CHROMATOGRAPHY A, 1996, 733 (1-2) : 143 - 157
[7] POLYCYCLIC AROMATIC-HYDROCARBONS IN SMOKED FOOD-PRODUCTS AND COMMERCIAL LIQUID SMOKE FLAVORINGS
GOMAA, EA
GRAY, JI
RABIE, S
LOPEZBOTE, C
BOOREN, AM
[J]. FOOD ADDITIVES AND CONTAMINANTS, 1993, 10 (05): : 503 - 521
[8] HERNANDEZ JE, 1995, B ENVIRON CONTAM TOX, V55, P461
[9] HISCHENHUBER C, 1987, DEUT LEBENSM-RUNDSCH, V83, P1
[10] Use of supercritical fluid extraction-high performance liquid chromatography in the determination of polynuclear aromatic hydrocarbons from smoked and broiled fish
Jarvenpaa, E
Huopalahti, R
Tapanainen, P
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1996, 19 (09) : 1473 - 1482

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