Biochemical analysis and hypoglycemic activity of a polysaccharide isolated from the fruit of Lycium barbarum L

被引：108
|
作者：
Tang, Hua-Li [1 ,2 ,3 ]
Chen, Chen [1 ,2 ]
Wang, Shao-Kang [1 ,2 ]
Sun, Gui-Ju [1 ,2 ]
机构：
[1] Southeast Univ, Sch Publ Hlth, Minist Educ, Key Lab Environm Med & Engn, Nanjing 210009, Jiangsu, Peoples R China
[2] Southeast Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Nanjing 210009, Jiangsu, Peoples R China
[3] Chongqing Three Gorges Univ, Sch Life Sci & Engn, Chongqing 404000, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 77卷
基金：
中国国家自然科学基金;
关键词：
Lycium barbarum L; Polysaccharide; Characterization; Hypoglycemic activity; STRUCTURAL-CHARACTERIZATION; EXTRACTS; RATS; OPTIMIZATION; EFFICACY; MICE; ROOT;
D O I：
10.1016/j.ijbiomac.2015.03.026
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Purification, characterization and hypoglycemic activities of polysaccharide from Lycium barbarum L. were investigated in this study. A water soluble polysaccharide (LBP) was obtained with ultrafiltration membranes separation, which was further purified by chromatography of DEAE cellulose column and Sephadex G-150 to get LBP3a and LBP3b. The high performance permeation chromatography (HPGPC) analysis showed that the average molecular weight (Mw) of LBP3b was 4.92 kDa. Monosaccharide composition analysis revealed that the LBP3b was comprised of mannose, rhamnose, glucose, galactose and xylose with a molar ratio of 5.52:5.11:28.06:1.00:1.70. The preliminary structure features of LBP3b were investigated by UV, FT-IR, NMR and SEM. In vitro cell experiments showed that LBP3b had significantly inhibited the absorption of glucose in a dose-dependent manner. The study showed that LBP3b had potential use as an anti-diabetic agent. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：235 / 242
页数：8
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