Constitutive heterochromatin heteromorphism in the Neotropical armored catfish Hypostomus regani (Ihering, 1905) (Loricariidae, Hypostominae) from the Paraguay River basin (Mato Grosso do Sul, Brazil)

被引:9
作者
de Brito Ferreira, Greicy Ellen [1 ]
Barbosa, Ligia Magrinelli [1 ]
Prizon-Nakajima, Ana Camila [1 ]
de Paiva, Suzana [1 ]
de Rossi Vieira, Margarida Maria [2 ]
Gallo, Raquel Bozini [3 ]
Borin-Carvalho, Luciana Andreia [1 ]
da Rosa, Renata [3 ]
Zawadzki, Claudio Henrique [4 ]
Martins dos Santos, Isabel Cristina [1 ]
de Brito Portela-Castro, Ana Luiza [1 ]
机构
[1] Univ Estadual Maringa, Dept Biotecnol Genet & Biol Celular, BR-87020900 Maringa, Parana, Brazil
[2] Univ Estadual Mato Grosso Sul, Ctr Ciencias Biol, BR-79400000 Coxim, MS, Brazil
[3] Univ Estadual Londrina, Dept Biol Geral, BR-86057970 Londrina, Parana, Brazil
[4] Univ Estadual Maringa, Dept Biol, Nucleo Pesquisas Limnol Ictiol & Aquicultura Nupe, BR-87020900 Maringa, Parana, Brazil
关键词
Chromosome painting; chromosomal polymorphism; chromosome specific probe; FISH; RIBOSOMAL-RNA GENES; 1803; SILURIFORMES; CYTOGENETIC CHARACTERIZATION; KARYOTYPIC DIVERSITY; CHROMOSOMAL DIVERSITY; SPECIES SILURIFORMES; 5S RDNA; LACEPEDE; 18S; TELEOSTEI;
D O I
10.3897/CompCytogen.v13i1.30134
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A cytogenetic analysis based on the integration of a number of different chromosomal methodologies, including chromosome microdissection was carried out to characterize the chromosomally polymorphic Hypostomus regani population from the Paraguay River basin, state of Mato Grosso do Sul in Brazil. All specimens had 2n=72 (FN=116) but two distinct karyotype formulas: karyomorph A (12m+14sm+18s+28a) and karyomorph B (13m+14sm+17st+28a). Karyomorph A and B differed only for pair 19 that consisted of two subtelocentrics in karyomorph A and a large metacentric and a subtelocentric in karyomorph B. This heteromorphism was due to extensive heterochromatinization of the short arm of the large metacentric, as highlighted by C-banding. The microdissection of the large metacentric of pair 19 allowed the production of a probe, named HrV (Hypostomus regani Variant), that hybridized to the whole p arm of the large metacentric and the pericentromeric region of the short arm of its (subtelocentric) homologue (karyomorph B) and of both homologs of pair 19 in karyomorph A. Additional cytogenetic techniques (FISH with 18S and 5S rDNA probes, CMA(3) and DAPI staining) allowed a finer distinction of the two karyomorphs. These results reinforced the hypothesis that the novel large metacentric of H. regani (karyomorph B) was the result of the amplification of heterochromatin segments, which contributed to karyotypic diversification in this species.
引用
收藏
页码:27 / 39
页数:13
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