Molecular cloning of mouse allantoicase cDNA

被引：7

作者：

Vigetti, D ^{[1
]}

Monetti, C ^{[1
]}

Bernardini, G ^{[1
]}

机构：

[1] Univ Insubria, Dipartimento Biol Strutturale & Funz, I-21100 Varese, Italy

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2001年 / 1519卷 / 1-2期

关键词：

purine degradation; evolution; uric acid; allantoin;

D O I：

10.1016/S0167-4781(01)00207-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The uric acid degradation pathway is progressively lost during vertebrate evolution. In mammals, the end product of this catabolic pathway is allantoin and, therefore, no allantoicase should be present in mouse tissues. Surprisingly, we have found an expressed sequence tag (EST) from mouse testis with high similarity to allantoicase. To characterize this transcript, we have completely sequenced the corresponding EST clone insert and found a 1495 bp long cDNA coding for a 414 amino acid long protein. Identities of mouse versus microorganism allantoicases range from 25 to 30%. Identity reaches 54% when compared to Xenopus allantoicase. Among the tested tissues, only testis possesses the allantoicase transcript. Although no deleterious mutations were found in the coding region, no allantoicase activity could be detected in mouse testis. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：117 / 121

页数：5

共 16 条

[1] Evolution of a metabolic pathway for degradation of a toxic xenobiotic: the patchwork approach [J].