Genome editing comes of age

被引：60

作者：

Kim, Jin-Soo ^{[1
,2
]}

机构：

[1] Inst for Basic Sci Korea, Ctr Genome Engn, Seoul, South Korea

[2] Seoul Natl Univ, Dept Chem, Seoul, South Korea

来源：

NATURE PROTOCOLS | 2016年 / 11卷 / 09期

关键词：

ZINC-FINGER NUCLEASES; CRISPR-CAS SYSTEM; RNA-GUIDED ENDONUCLEASES; DNA-BINDING SPECIFICITY; DOUBLE-STRAND BREAKS; OFF-TARGET ACTIVITY; HUMAN-CELLS; GENE KNOCKOUT; RESTRICTION-ENDONUCLEASE; CAENORHABDITIS-ELEGANS;

D O I：

10.1038/nprot.2016.104

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Genome editing harnesses programmable nucleases to cut and paste genetic information in a targeted manner in living cells and organisms. Here, I review the development of programmable nucleases, including zinc finger nucleases (ZFNs), TAL (transcription-activator-like) effector nucleases (TALENs) and CRISPR (cluster of regularly interspaced palindromic repeats)-Cas9 (CRISPR-associated protein 9) RNA-guided endonucleases (RGENs). I specifically highlight the key advances that set the foundation for the rapid and widespread implementation of CRISPR-Cas9 genome editing approaches that has revolutionized the field.

引用

页码：1573 / 1578

页数：6

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