Transcript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli

被引：21

作者：

Baek, Jong Hwan

Kang, Yeon Jae

Lee, Sang Yup

机构：

[1] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Metab & Biomol Engn Natl Res Lab, BK21 Program, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, BioProc Engn Res Ctr, Taejon 305701, South Korea

[3] Korea Adv Inst Sci & Technol, Ctr Syst & Synthet Biotechnol, Inst BioCentury, Taejon 305701, South Korea

[4] Korea Adv Inst Sci & Technol, Dept Biosyst & Bioinformat Res Ctr, Taejon 305701, South Korea

来源：

FEMS MICROBIOLOGY LETTERS | 2007年 / 277卷 / 02期

关键词：

Pho regulon; phosphate starvation response; protein-protein interaction; FRET; Escherichia coli;

D O I：

10.1111/j.1574-6968.2007.00965.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The responses to phosphate starvation by PhoR-PhoB two-component regulatory system are also affected by other regulatory systems in Escherichia coli. The interactions among PhoB, PhoR, PhoU and CreC were investigated at transcript and protein levels using real-time PCR and fluorescence resonance energy transfer analyses. CreC showed an interaction with PhoB, before an interaction between PhoB and PhoR, suggesting that this acts as a constant sensor for the early response to phosphate starvation. PhoU was found to interact with neither PhoB nor PhoR, while this acted as a phosphate starvation-inducible protein. These results showed the detailed time- and phosphate concentration-dependent interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in E. coli.

引用

页码：254 / 259

页数：6

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