Organic solvents-free technique for determining sulfadimethoxine and its metabolites in chicken meat

A quick and cost-effective technique of sample preparation followed by a reversed-phase high performance liquid chromatography under "organic solvent-free" (=100% aqueous) conditions for the simultaneous quantifying of sulfadimethoxine (SDM) and its metabolites, 6-hydroxy SDM (6-OH) and N-4-acetyl SDM (N-4-Ac), in chicken muscle is presented. Analysis by HPLC with photo-diode array detector was performed using a short Cl column with an isocratic 0.04 mol/l citric acid mobile phase. The method was validated by the analyses of spiked chicken muscle samples, resulting recoveries (<= 84%; relative standard deviations <= 6%), analytical total time (< 1/2h/sample, where a batch of 12 samples in 4 h), and limits of quantitation (<= 0. 1 mu g/g). The decision limits and detection capability were 0.019-0.106 and 0.054-0.112 mu g/g, respectively. No organic solvents were used at all. (c) 2007 Published by Elsevier B.V.