Identification of new partners of the epithelial sodium channel alpha subunit

被引：15
作者：
Malbert-Colas, L
Nicolas, G
Galand, C
Lecomte, MC
Dhermy, D
机构：
[1] Inst Claude Bernard, Fac Med Xavier Bichat, INSERM, U409, F-75780 Paris 18, France
[2] Inst Claude Bernard, Fac Med Xavier Bichat, IFR02, F-75780 Paris 18, France
来源：
COMPTES RENDUS BIOLOGIES | 2003年 / 326卷 / 07期
关键词：
alpha-ENaC subunit; Nedd4-2; WWP1; TSG101; UBC9; two-hybrid system;
D O I：
10.1016/S1631-0691(03)00154-9
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A fine regulation of the amiloride-sensitive Epithelial Sodium Channel (ENaC), made of alpha, beta and gamma subunits, is crucial for maintenance of Na+ balance and blood pressure. Both beta- and gamma-ENaC participate in negative regulation by interacting with Nedd4-2, an E3 ubiquitin-ligase. Disruption of this interaction results in increased ENaC activity (Liddle syndrome). By two-hybrid screenings, we identified new potential partners of alpha-ENaC: WWP1 (E3 ubiquitin-ligase protein), UBC9 and TSG101 (E2 ubiquitin/SUMO-conjugating enzymes) and confirmed these interactions in GST pull-down assays. All these partners are implicated in protein trafficking and could be involved in the regulation of ENaC activity. (C) 2003 Academie des sciences. Published by Editions scientifiques et medicales Elsevier SAS. All rights reserved.
引用
收藏
页码：615 / 624
页数：10
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