X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature

被引：12

作者：

Hutchison, Christopher D. M. ^{[1
]}

Cordon-Preciado, Violeta ^{[1
]}

Morgan, Rhodri M. L. ^{[2
]}

Nakane, Takanori ^{[3
]}

Ferreira, Josie ^{[1
]}

Dorlhiac, Gabriel ^{[1
]}

Sanchez-Gonzalez, Alvaro ^{[4
]}

Johnson, Allan S. ^{[4
]}

Fitzpatrick, Ann ^{[5
]}

Fare, Clyde ^{[1
]}

Marangos, Jon P. ^{[4
]}

Yoon, Chun Hong ^{[6
]}

Hunter, Mark S. ^{[6
]}

DePonte, Daniel P. ^{[6
]}

Boutet, Sebastien ^{[6
]}

Owada, Shigeki ^{[7
]}

Tanaka, Rie ^{[7
]}

Tono, Kensuke ^{[7
,8
]}

Iwata, So ^{[7
,9
]}

van Thor, Jasper J. ^{[1
]}

机构：

[1] Imperial Coll London, Mol Biophys, South Kensington Campus, London SW7 2AZ, England

[2] Imperial Coll London, Prot Crystallog Facil, Struct Biol Ctr, Dept Life Sci, Flowers Bldg, London SW7 2AZ, England

[3] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, 2-11-16 Yayoi, Tokyo 1130032, Japan

[4] Imperial Coll, Blackett Lab, Quantum Opt & Laser Sci Grp, London SW7 2AZ, England

[5] Diamond Light Source Ltd, Diamond House,Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England

[6] SLAC Natl Accelerator Lab, LCLS, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA

[7] RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan

[8] Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan

[9] Kyoto Univ, Grad Sch Med, Dept Cell Biol, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2017年 / 18卷 / 09期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会; 英国科学技术设施理事会; 欧洲研究理事会;

关键词：

XFEL; SFX; rsFP; Skylan-NS; SACLA; LCLS; ENERGY-TRANSFER; CRYSTALLOGRAPHY; REFINEMENT; PHOTOISOMERIZATION; DIFFRACTION; DRONPA; MODEL;

D O I：

10.3390/ijms18091918

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The photochromic fluorescent protein Skylan-NS (Nonlinear Structured illumination variant mEos3.1H62L) is a reversibly photoswitchable fluorescent protein which has an unilluminated/ground state with an anionic and cis chromophore conformation and high fluorescence quantum yield. Photo-conversion with illumination at 515 nm generates a meta-stable intermediate with neutral trans-chromophore structure that has a 4 h lifetime. We present X-ray crystal structures of the cis (on) state at 1.9 Angstrom resolution and the trans (off) state at a limiting resolution of 1.55 Angstrom from serial femtosecond crystallography experiments conducted at SPring-8 Angstrom Compact Free Electron Laser (SACLA) at 7.0 keV and 10.5 keV, and at Linac Coherent Light Source (LCLS) at 9.5 keV. We present a comparison of the data reduction and structure determination statistics for the two facilities which differ in flux, beam characteristics and detector technologies. Furthermore, a comparison of droplet on demand, grease injection and Gas Dynamic Virtual Nozzle (GDVN) injection shows no significant differences in limiting resolution. The photoconversion of the on- to the off-state includes both internal and surface exposed protein structural changes, occurring in regions that lack crystal contacts in the orthorhombic crystal form.

引用

页数：16

共 65 条

[51] Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein [J].

Tenboer, Jason ;

Basu, Shibom ;

Zatsepin, Nadia ;

Pande, Kanupriya ;

Milathianaki, Despina ;

Frank, Matthias ;

Hunter, Mark ;

Boutet, Sebastien ;

Williams, Garth J. ;

Koglin, Jason E. ;

Oberthuer, Dominik ;

Heymann, Michael ;

Kupitz, Christopher ;

Conrad, Chelsie ;

Coe, Jesse ;

Roy-Chowdhury, Shatabdi ;

Weierstall, Uwe ;

James, Daniel ;

Wang, Dingjie ;

Grant, Thomas ;

Barty, Anton ;

Yefanov, Oleksandr ;

Scales, Jennifer ;

Gati, Cornelius ;

Seuring, Carolin ;

Srajer, Vukica ;

Henning, Robert ;

Schwander, Peter ;

Fromme, Raimund ;

Ourmazd, Abbas ;

Moffat, Keith ;

Van Thor, Jasper J. ;

Spence, John C. H. ;

Fromme, Petra ;

Chapman, Henry N. ;

Schmidt, Marius .

SCIENCE, 2014, 346 (6214) :1242-1246

[52] Data systems for the Linac Coherent Light Source [J].

Thayer, J. ;

Damiani, D. ;

Ford, C. ;

Gaponenko, I. ;

Kroeger, W. ;

O'Grady, C. ;

Pines, J. ;

Tookey, T. ;

Weaver, M. ;

Perazzo, A. .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2016, 49 :1363-1369

[53] Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser [J].

Tono, Kensuke ;

Nango, Eriko ;

Sugahara, Michihiro ;

Song, Changyong ;

Park, Jaehyun ;

Tanaka, Tomoyuki ;

Tanaka, Rie ;

Joti, Yasumasa ;

Kameshima, Takashi ;

Ono, Shun ;

Hatsui, Takaki ;

Mizohata, Eiichi ;

Suzuki, Mamoru ;

Shimamura, Tatsuro ;

Tanaka, Yoshiki ;

Iwata, So ;

Yabashi, Makina .

JOURNAL OF SYNCHROTRON RADIATION, 2015, 22 :532-537

[54] Enabling X-ray Free Electron Laser Crystallography for Challenging Biological Systems from a Limited Number of Crystals [J].

Uervirojnangkoorn, Monarin ;

Zeldin, Oliver B. ;

Lyubimov, Artem Y. ;

Hattne, Johan ;

Brewster, Aaron S. ;

Sauter, Nicholas K. ;

Brunger, Axel T. ;

Weis, William I. .

ELIFE, 2015, 4

[55] MOLREP: an automated program for molecular replacement [J].

Vagin, A ;

Teplyakov, A .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1997, 30 :1022-1025

[56] Signal to noise considerations for single crystal femtosecond time resolved crystallography of the Photoactive Yellow Protein [J].

van Thor, Jasper J. ;

Warren, Mark M. ;

Lincoln, Craig N. ;

Chollet, Matthieu ;

Lemke, Henrik Till ;

Fritz, David M. ;

Schmidt, Marius ;

Tenboer, Jason ;

Ren, Zhong ;

Srajer, Vukica ;

Moffat, Keith ;

Graber, Tim .

FARADAY DISCUSSIONS, 2014, 171 :439-455

[57] SPECTROPHOTOMETRIC IDENTITY OF THE ENERGY-TRANSFER CHROMOPHORES IN RENILLA AND AEQUOREA GREEN-FLUORESCENT PROTEINS [J].

WARD, WW ;

CODY, CW ;

HART, RC ;

CORMIER, MJ .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1980, 31 (06) :611-615

[58] Ground-state proton transfer in the photoswitching reactions of the fluorescent protein Dronpa [J].

Warren, Mark M. ;

Kaucikas, Marius ;

Fitzpatrick, Ann ;

Champion, Paul ;

Sage, J. Timothy ;

van Thor, Jasper J. .

NATURE COMMUNICATIONS, 2013, 4

[59] Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography [J].

Weierstall, Uwe ;

James, Daniel ;

Wang, Chong ;

White, Thomas A. ;

Wang, Dingjie ;

Liu, Wei ;

Spence, John C. H. ;

Doak, R. Bruce ;

Nelson, Garrett ;

Fromme, Petra ;

Fromme, Raimund ;

Grotjohann, Ingo ;

Kupitz, Christopher ;

Zatsepin, Nadia A. ;

Liu, Haiguang ;

Basu, Shibom ;

Wacker, Daniel ;

Han, Gye Won ;

Katritch, Vsevolod ;

Boutet, Sebastien ;

Messerschmidt, Marc ;

Williams, Garth J. ;

Koglin, Jason E. ;

Seibert, M. Marvin ;

Klinker, Markus ;

Gati, Cornelius ;

Shoeman, Robert L. ;

Barty, Anton ;

Chapman, Henry N. ;

Kirian, Richard A. ;

Beyerlein, Kenneth R. ;

Stevens, Raymond C. ;

Li, Dianfan ;

Shah, Syed T. A. ;

Howe, Nicole ;

Caffrey, Martin ;

Cherezov, Vadim .

NATURE COMMUNICATIONS, 2014, 5

[60] Post-refinement method for snapshot serial crystallography [J].

White, Thomas A. .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2014, 369 (1647)

← 1 2 3 4 5 6 7 →