X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature

被引：12

作者：

Hutchison, Christopher D. M. ^{[1
]}

Cordon-Preciado, Violeta ^{[1
]}

Morgan, Rhodri M. L. ^{[2
]}

Nakane, Takanori ^{[3
]}

Ferreira, Josie ^{[1
]}

Dorlhiac, Gabriel ^{[1
]}

Sanchez-Gonzalez, Alvaro ^{[4
]}

Johnson, Allan S. ^{[4
]}

Fitzpatrick, Ann ^{[5
]}

Fare, Clyde ^{[1
]}

Marangos, Jon P. ^{[4
]}

Yoon, Chun Hong ^{[6
]}

Hunter, Mark S. ^{[6
]}

DePonte, Daniel P. ^{[6
]}

Boutet, Sebastien ^{[6
]}

Owada, Shigeki ^{[7
]}

Tanaka, Rie ^{[7
]}

Tono, Kensuke ^{[7
,8
]}

Iwata, So ^{[7
,9
]}

van Thor, Jasper J. ^{[1
]}

机构：

[1] Imperial Coll London, Mol Biophys, South Kensington Campus, London SW7 2AZ, England

[2] Imperial Coll London, Prot Crystallog Facil, Struct Biol Ctr, Dept Life Sci, Flowers Bldg, London SW7 2AZ, England

[3] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, 2-11-16 Yayoi, Tokyo 1130032, Japan

[4] Imperial Coll, Blackett Lab, Quantum Opt & Laser Sci Grp, London SW7 2AZ, England

[5] Diamond Light Source Ltd, Diamond House,Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England

[6] SLAC Natl Accelerator Lab, LCLS, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA

[7] RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan

[8] Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan

[9] Kyoto Univ, Grad Sch Med, Dept Cell Biol, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2017年 / 18卷 / 09期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会; 欧洲研究理事会; 英国科学技术设施理事会;

关键词：

XFEL; SFX; rsFP; Skylan-NS; SACLA; LCLS; ENERGY-TRANSFER; CRYSTALLOGRAPHY; REFINEMENT; PHOTOISOMERIZATION; DIFFRACTION; DRONPA; MODEL;

D O I：

10.3390/ijms18091918

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The photochromic fluorescent protein Skylan-NS (Nonlinear Structured illumination variant mEos3.1H62L) is a reversibly photoswitchable fluorescent protein which has an unilluminated/ground state with an anionic and cis chromophore conformation and high fluorescence quantum yield. Photo-conversion with illumination at 515 nm generates a meta-stable intermediate with neutral trans-chromophore structure that has a 4 h lifetime. We present X-ray crystal structures of the cis (on) state at 1.9 Angstrom resolution and the trans (off) state at a limiting resolution of 1.55 Angstrom from serial femtosecond crystallography experiments conducted at SPring-8 Angstrom Compact Free Electron Laser (SACLA) at 7.0 keV and 10.5 keV, and at Linac Coherent Light Source (LCLS) at 9.5 keV. We present a comparison of the data reduction and structure determination statistics for the two facilities which differ in flux, beam characteristics and detector technologies. Furthermore, a comparison of droplet on demand, grease injection and Gas Dynamic Virtual Nozzle (GDVN) injection shows no significant differences in limiting resolution. The photoconversion of the on- to the off-state includes both internal and surface exposed protein structural changes, occurring in regions that lack crystal contacts in the orthorhombic crystal form.

引用

页数：16

共 65 条

[1] Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations [J].

Adam, Virgile ;

Lelimousin, Mickal ;

Boehme, Susan ;

Desfonds, Guillaume ;

Nienhaus, Karin ;

Field, Martin J. ;

Wiedenmann, Joerg ;

McSweeney, Sean ;

Nienhaus, G. Ulrich ;

Bourgeois, Dominique .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (47) :18343-18348

[2] NEW LEAST-SQUARES REFINEMENT TECHNIQUE BASED ON FAST FOURIER-TRANSFORM ALGORITHM [J].

AGARWAL, RC .

ACTA CRYSTALLOGRAPHICA SECTION A, 1978, 34 (SEP) :791-809

[3] Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting [J].

Ando, R ;

Mizuno, H ;

Miyawaki, A .

SCIENCE, 2004, 306 (5700) :1370-1373

[4] Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy [J].

Andresen, Martin ;

Stiel, Andre C. ;

Foelling, Jonas ;

Wenzel, Dirk ;

Schoenle, Andreas ;

Egner, Alexander ;

Eggeling, Christian ;

Hell, Stefan W. ;

Jakobs, Stefan .

NATURE BIOTECHNOLOGY, 2008, 26 (09) :1035-1040

[5] Structural basis for reversible photoswitching in Dronpa [J].

Andresen, Martin ;

Stiel, Andre C. ;

Trowitzsch, Simon ;

Weber, Gert ;

Eggeling, Christian ;

Wahl, Markus C. ;

Hell, Stefan W. ;

Jakobs, Stefan .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (32) :13005-13009

[6] Effects of self-seeding and crystal post-selection on the quality of Monte Carlo-integrated SFX data [J].

Barends, Thomas ;

White, Thomas A. ;

Barty, Anton ;

Foucar, Lutz ;

Messerschmidt, Marc ;

Alonso-Mori, Roberto ;

Botha, Sabine ;

Chapman, Henry ;

Doak, R. Bruce ;

Galli, Lorenzo ;

Gati, Cornelius ;

Gutmann, Matthias ;

Koglin, Jason ;

Markvardsen, Anders ;

Nass, Karol ;

Oberthur, Dominik ;

Shoeman, Robert L. ;

Schlichting, Ilme ;

Boutet, Sebastien .

JOURNAL OF SYNCHROTRON RADIATION, 2015, 22 :644-652

[7] Cheetah: software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data [J].

Barty, Anton ;

Kirian, Richard A. ;

Maia, Filipe R. N. C. ;

Hantke, Max ;

Yoon, Chun Hong ;

White, Thomas A. ;

Chapman, Henry .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2014, 47 :1118-1131

[8] High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography [J].

Boutet, Sebastien ;

Lomb, Lukas ;

Williams, Garth J. ;

Barends, Thomas R. M. ;

Aquila, Andrew ;

Doak, R. Bruce ;

Weierstall, Uwe ;

DePonte, Daniel P. ;

Steinbrener, Jan ;

Shoeman, Robert L. ;

Messerschmidt, Marc ;

Barty, Anton ;

White, Thomas A. ;

Kassemeyer, Stephan ;

Kirian, Richard A. ;

Seibert, M. Marvin ;

Montanez, Paul A. ;

Kenney, Chris ;

Herbst, Ryan ;

Hart, Philip ;

Pines, Jack ;

Haller, Gunther ;

Gruner, Sol M. ;

Philipp, Hugh T. ;

Tate, Mark W. ;

Hromalik, Marianne ;

Koerner, Lucas J. ;

van Bakel, Niels ;

Morse, John ;

Ghonsalves, Wilfred ;

Arnlund, David ;

Bogan, Michael J. ;

Caleman, Carl ;

Fromme, Raimund ;

Hampton, Christina Y. ;

Hunter, Mark S. ;

Johansson, Linda C. ;

Katona, Gergely ;

Kupitz, Christopher ;

Liang, Mengning ;

Martin, Andrew V. ;

Nass, Karol ;

Redecke, Lars ;

Stellato, Francesco ;

Timneanu, Nicusor ;

Wang, Dingjie ;

Zatsepin, Nadia A. ;

Schafer, Donald ;

Defever, James ;

Neutze, Richard .

SCIENCE, 2012, 337 (6092) :362-364

[9] Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein [J].

Brejc, K ;

Sixma, TK ;

Kitts, PA ;

Kain, SR ;

Tsien, RY ;

Ormo, M ;

Remington, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2306-2311

[10] A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications [J].

Chang, Hao ;

Zhang, Mingshu ;

Ji, Wei ;

Chen, Juanjuan ;

Zhang, Yongdeng ;

Liu, Bei ;

Lu, Jingze ;

Zhang, Junlong ;

Xu, Pingyong ;

Xu, Tao .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (12) :4455-4460

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