Gateway Vectors for Simultaneous Detection of Multiple Protein-Protein Interactions in Plant Cells Using Bimolecular Fluorescence Complementation

被引:22
作者
Kamigaki, Akane [1 ]
Nito, Kazumasa [2 ]
Hikino, Kazumi [1 ]
Goto-Yamada, Shino [2 ,6 ]
Nishimura, Mikio [3 ,7 ]
Nakagawa, Tsuyoshi [4 ]
Mano, Shoji [1 ,5 ]
机构
[1] Natl Inst Basic Biol, Dept Evolutionary Biol & Biodivers, Okazaki, Aichi, Japan
[2] Kyoto Univ, Grad Sch Sci, Kyoto, Japan
[3] Natl Inst Basic Biol, Dept Cell Biol, Okazaki, Aichi, Japan
[4] Shimane Univ, Dept Mol & Funct Genom, Interdisciplinary Ctr Sci Res, Org Res, Matsue, Shimane, Japan
[5] SOKENDAI Grad Univ Adv Studies, Sch Life Sci, Dept Basic Biol, Okazaki, Aichi, Japan
[6] Jagiellonian Univ, Malopolska Ctr Biotechnol, Krakow, Poland
[7] Natl Inst Basic Biol, Res Enhancement Strategy Off, Okazaki, Aichi, Japan
来源
PLOS ONE | 2016年 / 11卷 / 08期
关键词
BINARY VECTORS; LIVING CELLS; SIMULTANEOUS VISUALIZATION; PHYSIOLOGICAL CONDITIONS; ARABIDOPSIS-THALIANA; IN-PLANTA; RED; PEROXISOMES; SYSTEM; COMPLEXES;
D O I
10.1371/journal.pone.0160717
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bimolecular fluorescence complementation (BiFC) is widely used to detect protein-protein interactions, because it is technically simple, convenient, and can be adapted for use with conventional fluorescence microscopy. We previously constructed enhanced yellow fluorescent protein (EYFP)-based Gateway cloning technology-compatible vectors. In the current study, we generated new Gateway cloning technology-compatible vectors to detect BiFC-based multiple protein-protein interactions using N- and C-terminal fragments of enhanced cyan fluorescent protein (ECFP), enhanced green fluorescent protein (EGFP), and monomeric red fluorescent protein (mRFP1). Using a combination of N- and C-terminal fragments from ECFP, EGFP and EYFP, we observed a shift in the emission wavelength, enabling the simultaneous detection of multiple protein-protein interactions. Moreover, we developed these vectors as binary vectors for use in Agrobacterium infiltration and for the generate transgenic plants. We verified that the binary vectors functioned well in tobacco cells. The results demonstrate that the BiFC vectors facilitate the design of various constructions and are convenient for the detection of multiple protein-protein interactions simultaneously in plant cells.
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页数:15
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共 36 条
[1]   IAA8 Involved in Lateral Root Formation Interacts with the TIR1 Auxin Receptor and ARF Transcription Factors in Arabidopsis [J].
Arase, Fumi ;
Nishitani, Hiroko ;
Egusa, Mayumi ;
Nishimoto, Nami ;
Sakurai, Sumiko ;
Sakamoto, Naho ;
Kaminaka, Hironori .
PLOS ONE, 2012, 7 (08)
[2]   Circular permutation and receptor insertion within green fluorescent proteins [J].
Baird, GS ;
Zacharias, DA ;
Tsien, RY .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11241-11246
[3]   Detection of protein-protein interactions in plants using bimolecular fluorescence complementation [J].
Bracha-Drori, K ;
Shichrur, K ;
Katz, A ;
Oliva, M ;
Angelovici, R ;
Yalovsky, S ;
Ohad, N .
PLANT JOURNAL, 2004, 40 (03) :419-427
[4]   A novel far-red bimolecular fluorescence complementation system that allows for efficient visualization of protein interactions under physiological conditions [J].
Chu, Jun ;
Zhang, Zhihong ;
Zheng, Ying ;
Yang, Jie ;
Qin, Lingsong ;
Lu, Jinling ;
Huang, Zhen-Li ;
Zeng, Shaoqun ;
Luo, Qingming .
BIOSENSORS & BIOELECTRONICS, 2009, 25 (01) :234-239
[5]   Localizing protein-protein interactions by bimolecular fluorescence complementation in planta [J].
Citovsky, Vitaly ;
Gafni, Yedidya ;
Tzfira, Tzvi .
METHODS, 2008, 45 (03) :196-206
[6]   The DOF protein, SAD, interacts with GAMYB in plant nuclei and activates transcription of endosperm-specific genes during barley seed development [J].
Diaz, I ;
Martinez, M ;
Isabel-LaMoneda, I ;
Rubio-Somoza, I ;
Carbonero, P .
PLANT JOURNAL, 2005, 42 (05) :652-662
[7]   Arabidopsis GLUTAMINE-RICH PROTEIN23 is essential for early embryogenesis and encodes a novel nuclear PPR motif protein that interacts with RNA polymerase II subunit III [J].
Ding, YH ;
Liu, NY ;
Tang, ZS ;
Liu, J ;
Yang, WC .
PLANT CELL, 2006, 18 (04) :815-830
[8]   Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells [J].
Fan, Jin-Yu ;
Cui, Zong-Qiang ;
Wei, Hong-Ping ;
Zhang, Zhi-Ping ;
Zhou, Ya-Feng ;
Wang, Yun-Peng ;
Zhang, Xian-En .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 367 (01) :47-53
[9]   Arabidopsis ABERRANT PEROXISOME MORPHOLOGY9 Is a Peroxin That Recruits the PEX1-PEX6 Complex to Peroxisomes [J].
Goto, Shino ;
Mano, Shoji ;
Nakamori, Chihiro ;
Nishimura, Mikio .
PLANT CELL, 2011, 23 (04) :1573-1587
[10]   Arabidopsis TERMINAL FLOWER1 Is Involved in the Regulation of Flowering Time and Inflorescence Development through Transcriptional Repression [J].
Hanano, Shigeru ;
Goto, Koji .
PLANT CELL, 2011, 23 (09) :3172-3184