A novel long non-coding RNA NFIA-AS1 is down-regulated in gastric cancer and inhibits proliferation of gastric cancer cells

被引:1
作者
Wang, Jing [1 ,2 ,3 ]
He, Xuezhi [1 ,2 ,3 ]
Lu, Xiyi [4 ]
Karim, Muhammad Amin [1 ,2 ,3 ]
Miao, Dengshun [1 ,2 ,3 ]
Zhang, Erbao [5 ]
机构
[1] Nanjing Med Univ, Res Ctr Bone & Stem Cells, Dept Anat, Nanjing 211166, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Res Ctr Bone & Stem Cells, Dept Histol, Nanjing 211166, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Res Ctr Bone & Stem Cells, Dept Embryol, Nanjing 211166, Jiangsu, Peoples R China
[4] Nanjing Med Univ, Dept Oncol, Affiliated Hosp 1, Nanjing 210009, Jiangsu, Peoples R China
[5] Nanjing Med Univ, Dept Epidemiol & Biostat, Jiangsu Key Lab Canc Biomarkers Prevent & Treatme, Collaborat Innovat Ctr Canc Personalized Med, Nanjing 211166, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
gastric cancer; long non-coding RNA; NFIA antisense RNA 1 (NFIA-AS1); cell proliferation; METASTASIS; EXPRESSION; LNCRNA; PROGNOSIS; GROWTH;
D O I
10.7555/JBR.33.20190015
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Gastric cancer is one of the most common malignant gastrointestinal tumors whose morbidity and mortality account for the second and third place respectively in malignant tumors in China. As an important participant in tumor biology, the abnormal expression of long non-coding RNA (lncRNAs) in cancer cells is closely related to the occurrence and development of tumors and plays the role of oncogenes or tumor suppressor genes. In this study, we identified a novel lncRNA NFIA antisense RNA 1 (NFIA-AS1) and explored its role and clinical significance in gastric cancer. Real-time quantitative PCR was performed to detect the expression of NFIA-AS1 in tumor tissues and corresponding normal tissues from 42 pairs of gastric cancer samples. The lower expression of NFIA-AS1 was significantly associated with larger tumor size, lower histological grade, and advanced TNM stage. Kaplan-meier analysis showed that NFIA-AS1 expression could be used as an independent predictor of overall survival. We also demonstrated that overexpression of NFIA-AS1 significantly inhibited the proliferation of gastric cancer cells through affecting p16 levels. In conclusion, our results suggest that the lncRNA NFIA-AS1 may play the role of tumor suppressor gene, and serve as a biomarker for prognosis or progression of gastric cancer.
引用
收藏
页码:371 / 381
页数:11
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