Stability of a black eyed pea trypsin/chymotrypsin inhibitor (BTCI)

被引：20
作者：
da Silva, LP
Leite, JRSA
Bloch, C
de Freitas, SM
机构：
[1] Univ Brasilia, Dept Biol Celular, Brasilia, DF, Brazil
[2] EMBRAPA, CENARGEN, Lab Espectrometria Massa, Brasilia, DF, Brazil
[3] Univ Brasilia, Inst Quim, Brasilia, DF, Brazil
来源：
PROTEIN AND PEPTIDE LETTERS | 2001年 / 8卷 / 01期
关键词：
Bowman-Birk inhibitor; thermal stability; trypsin inhibitor; Vigna unguiculata;
D O I：
10.2174/0929866013409715
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The stability of BTCI has been investigated as function of pH and temperature, following its inhibitory activity against trypsin. The isolated inhibitor of 9,084 Da is stable over pH 3 to 10 at 25 degreesC. BTCI showed high thermal stability ranging from 25 to 95 degreesC at pH 3.0 and 7.0. However, the protein lost about 20% of its inhibitory activity over 75 degreesC at pH 8.2. The results indicated that BTCI is extremely stable to heat and pH as typical of Bowman-Birk inhibitors.
引用
收藏
页码：33 / 38
页数：6
相关论文
共 23 条
[1]  
BIRK Y, 1987, HYDROLYTIC ENZYMES, P257
[2]   Dimeric crystal structure of a Bowman-Birk protease inhibitor from pea seeds [J].
de la Sierra, IL ;
Quillien, L ;
Flecker, P ;
Gueguen, J ;
Brunie, S .
JOURNAL OF MOLECULAR BIOLOGY, 1999, 285 (03) :1195-1207
[3]   Analysis of the black-eyed pea trypsin and chymotrypsin inhibitor alpha-chymotrypsin complex [J].
deFreitas, SM ;
deMello, LV ;
daSilva, MCM ;
Vriend, G ;
Neshich, G ;
Ventura, MM .
FEBS LETTERS, 1997, 409 (02) :121-127
[4]   Purification and characterization of a proteinase inhibitor from field bean, Dolichos lablab perpureus L. [J].
Devaraj, VR ;
Manjunatha, NH .
JOURNAL OF PROTEIN CHEMISTRY, 1999, 18 (01) :47-54
[5]   BOWMAN-BIRK PROTEINASE-INHIBITOR (BBI) MODULATES RADIOSENSITIVITY AND RADIATION-INDUCED DIFFERENTIATION OF HUMAN FIBROBLASTS IN CULTURE [J].
DITTMANN, K ;
LOFFLER, H ;
BAMBERG, M ;
RODEMANN, HP .
RADIOTHERAPY AND ONCOLOGY, 1995, 34 (02) :137-143
[6]   PREPARATION AND PROPERTIES OF 2 NEW CHROMOGENIC SUBSTRATES OF TRYPSIN [J].
ERLANGER, BF ;
COHEN, W ;
KOKOWSKY, N .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1961, 95 (02) :271-&
[7]  
FACHETTI HCS, 1984, AN ACAD BRAS CIENC, V56, P311
[8]  
FREITAS SM, 1999, J PROTEIN CHEM, V18, P307
[9]   ISOLATION AND CHARACTERIZATION OF TRYPSIN-INHIBITORS FROM COWPEA (VIGNA-UNGUICULATA) [J].
GATEHOUSE, AMR ;
GATEHOUSE, JA ;
BOULTER, D .
PHYTOCHEMISTRY, 1980, 19 (05) :751-756
[10]   A NOVEL MECHANISM OF INSECT RESISTANCE ENGINEERED INTO TOBACCO [J].
HILDER, VA ;
GATEHOUSE, AMR ;
SHEERMAN, SE ;
BARKER, RF ;
BOULTER, D .
NATURE, 1987, 330 (6144) :160-163
123