A Mouse Model of Zika Virus Pathogenesis

被引：737

作者：

Lazear, Helen M. ^{[1
]}

Govero, Jennifer ^{[2
]}

Smith, Amber M. ^{[2
]}

Platt, Derek J. ^{[2
]}

Fernandez, Estefania ^{[2
]}

Miner, Jonathan J. ^{[2
]}

Diamond, Michael S. ^{[2
,3
,4
,5
]}

机构：

[1] Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA

[2] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA

[3] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

[4] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA

[5] Washington Univ, Sch Med, Ctr Human Immunol & Immunotherapy Programs, St Louis, MO 63110 USA

来源：

CELL HOST & MICROBE | 2016年 / 19卷 / 05期

关键词：

UNITED-STATES; ALPHA/BETA; TRANSMISSION; INFECTION; ISOLATIONS; OUTBREAK; PROTEIN; BRAZIL; ROLES; BRAIN;

D O I：

10.1016/j.chom.2016.03.010

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The ongoing Zika virus (ZIKV) epidemic and unexpected clinical outcomes, including Guillain-Barre syndrome and birth defects, has brought an urgent need for animal models. We evaluated infection and pathogenesis with contemporary and historical ZIKV strains in immunocompetent mice and mice lacking components of the antiviral response. Four-to six-week-old Irf3(-/-) Irf5(-/-) Irf7(-/-) triple knockout mice, which produce little interferon alpha/beta, and mice lacking the interferon receptor (Ifnar1(-/-)) developed neurological disease and succumbed to ZIKV infection, whereas single Irf3(-/-), Irf5(-/-), and Mavs(-/-) knockout mice exhibited no overt illness. Ifnar1(-/-) mice sustained high viral loads in the brain and spinal cord, consistent with evidence that ZIKV causes neurodevelopmental defects in human fetuses. The testes of Ifnar1(-/-) mice had the highest viral loads, which is relevant to sexual transmission of ZIKV. This model of ZIKV pathogenesis will be valuable for evaluating vaccines and therapeutics as well as understanding disease pathogenesis.

引用

页码：720 / 730

页数：11

共 67 条

[1] Ecuador Paraiso Escondido Virus, a New Flavivirus Isolated from New World Sand Flies in Ecuador, Is the First Representative of a Novel Clade in the Genus Flavivirus [J].

Alkan, Cigdem ;

Zapata, Sonia ;

Bichaud, Laurence ;

Moureau, Gregory ;

Lemey, Philippe ;

Firth, Andrew E. ;

Gritsun, Tamara S. ;

Gould, Ernest A. ;

de Lamballerie, Xavier ;

Depaquit, Jerome ;

Charrel, Remi N. .

JOURNAL OF VIROLOGY, 2015, 89 (23) :11773-11785

[2] An important role for type III interferon (IFN-λ/IL-28) in TLR-induced antiviral activity [J].

Ank, Nina ;

Iversen, Marie B. ;

Bartholdy, Christina ;

Staeheli, Peter ;

Hartmann, Rune ;

Jensen, Uffe B. ;

Dagnaes-Hansen, Frederik ;

Thomsen, Allan R. ;

Chen, Zhi ;

Haugen, Harald ;

Klucher, Kevin ;

Paludan, Soren R. .

JOURNAL OF IMMUNOLOGY, 2008, 180 (04) :2474-2485

[3] Detection of Zika Virus in Semen [J].

Atkinson, Barry ;

Hearn, Pasco ;

Afrough, Babak ;

Lumley, Sarah ;

Carter, Daniel ;

Aarons, Emma J. ;

Simpson, Andrew J. ;

Brooks, Timothy J. ;

Hewson, Roger .

EMERGING INFECTIOUS DISEASES, 2016, 22 (05) :940-940

[4] Complete Coding Sequence of Zika Virus from a French Polynesia Outbreak in 2013 [J].

Baronti, Cecile ;

Piorkowski, Geraldine ;

Charrel, Remi N. ;

Boubis, Laetitia ;

Leparc-Goffart, Isabelle ;

de Lamballerie, Xavier .

GENOME ANNOUNCEMENTS, 2014, 2 (03)

[5] ZIKA VIRUS INFECTION OF CENTRAL NERVOUS SYSTEM OF MICE [J].

BELL, TM ;

FIELD, EJ ;

NARANG, HK .

ARCHIV FUR DIE GESAMTE VIRUSFORSCHUNG, 1971, 35 (2-3) :183-+

[6] Zika Virus Infection in Pregnant Women in Rio de Janeiro [J].

Brasil, P. ;

Pereira, J. P., Jr. ;

Moreira, M. E. ;

Ribeiro Nogueira, R. M. ;

Damasceno, L. ;

Wakimoto, M. ;

Rabello, R. S. ;

Valderramos, S. G. ;

Halai, U. -A. ;

Salles, T. S. ;

Zin, A. A. ;

Horovitz, D. ;

Daltro, P. ;

Boechat, M. ;

Gabaglia, C. Raja ;

Carvalho de Sequeira, P. ;

Pilotto, J. H. ;

Medialdea-Carrera, R. ;

Cotrim da Cunha, D. ;

Abreu de Carvalho, L. M. ;

Pone, M. ;

Machado Siqueira, A. ;

Calvet, G. A. ;

Rodrigues Baiao, A. E. ;

Neves, E. S. ;

Nassar de Carvalho, P. R. ;

Hasue, R. H. ;

Marschik, P. B. ;

Einspieler, C. ;

Janzen, C. ;

Cherry, J. D. ;

Bispo de Filippis, A. M. ;

Nielsen-Saines, K. .

NEW ENGLAND JOURNAL OF MEDICINE, 2016, 375 (24) :2321-2334

[7]

Brien James D, 2013, Curr Protoc Microbiol, V31, DOI 10.1002/9780471729259.mc15d03s31

[8] Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study [J].

Calvet, Guilherme ;

Aguiar, Renato S. ;

Melo, Adriana S. O. ;

Sampaio, Simone A. ;

de Filippis, Ivano ;

Fabri, Allison ;

Araujo, Eliane S. M. ;

de Sequeira, Patricia C. ;

de Mendonca, Marcos C. L. ;

de Oliveira, Louisi ;

Tschoeke, Diogo A. ;

Schrago, Carlos G. ;

Thompson, Fabiano L. ;

Brasil, Patricia ;

dos Santos, Flavia B. ;

Nogueira, Rita M. R. ;

Tanurit, Amilcar ;

de Filippis, Ana M. B. .

LANCET INFECTIOUS DISEASES, 2016, 16 (06) :653-660

[9] Guillain-Barre Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study [J].

Cao-Lormeau, Van-Mai ;

Blake, Alexandre ;

Mons, Sandrine ;

Lastere, Stephane ;

Roche, Claudine ;

Vanhomwegen, Jessica ;

Dub, Timothee ;

Baudouin, Laure ;

Teissier, Anita ;

Larre, Philippe ;

Vial, Anne-Laure ;

Decam, Christophe ;

Choumet, Valerie ;

Halstead, Susan K. ;

Willison, Hugh J. ;

Musset, Lucile ;

Manuguerra, Jean-Claude ;

Despres, Philippe ;

Fournier, Emmanuel ;

Mallet, Henri-Pierre ;

Musso, Didier ;

Fontanet, Arnaud ;

Neil, Jean ;

Ghawche, Frederic .

LANCET, 2016, 387 (10027) :1531-1539

[10]

Cao-Lormeau VM, 2014, EMERG INFECT DIS, V20, P1085, DOI [10.3201/eid2006.140138, 10.3201/eid2011.141380]

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