The enrichment of trophoblast giant cells from mid-gestation bovine placentae using fluorescence-activated cell sorting

被引：2
|
作者：
Pennington, K. A. [1 ]
Oliveira, L. J. [1 ]
Yang, Q. E. [1 ]
Ealy, A. D. [1 ]
机构：
[1] Univ Florida, Dept Anim Sci, DH Barron Reprod & Perinatal Biol Res Program, Gainesville, FL 32611 USA
来源：
PLACENTA | 2010年 / 31卷 / 08期
关键词：
Trophoblast; Pregnancy; Bos taurus; Binucleate cells; INTERFERON-TAU EXPRESSION; GROWTH-FACTORS; DIFFERENTIATION; PREGNANCY; RUMINANTS; LACTOGEN; CULTURE; GENES;
D O I：
10.1016/j.placenta.2010.05.008
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The objective of this work was to determine if fluorescence-activated cell sorting (FACS) can be used to isolate trophoblast giant cells (TGCs) from bovine placentae. Cotyledons were harvested, minced and digested with dispase/pancreatin. Tissue homogenates were incubated with Vybrant Dye Cycle-Green. FACS was completed, and cells with DNA content 2- to 4-times greater than cells within the diploid peak contained between 65 and 84% TGCs. The relative abundance of CSH1 and PAG1 transcripts were greater (P < 0.05) in TGC-enriched fractions than cells within the diploid peak. These observations indicate that FACS can effectively enrich TGCs from bovine placentae. (c) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：738 / 740
页数：3
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