Applications of Nisin and EDTA in Food Packaging for Improving Fabricated Chitosan-Polylactate Plastic Film Performance and Fish Fillet Preservation

被引:27
作者
Chang, Shun-Hsien [1 ]
Chen, Ying-Ju [2 ]
Tseng, Hsiang-Jung [3 ]
Hsiao, Hsin-I [2 ]
Chai, Huey-Jine [4 ]
Shang, Kuo-Chung [5 ]
Pan, Chorng-Liang [2 ]
Tsai, Guo-Jane [2 ,6 ]
机构
[1] Natl Taiwan Ocean Univ, Inst Food Safety & Risk Management, Keelung 202301, Taiwan
[2] Natl Taiwan Ocean Univ, Dept Food Sci, Keelung 202301, Taiwan
[3] Plast Ind Dev Ctr, Dept Res & Dev, Taichung 40768, Taiwan
[4] Fisher Res Inst, Seafood Technol Div, Council Agr, Keelung 202008, Taiwan
[5] Natl Taiwan Ocean Univ, Dept Transportat Sci, Keelung 202301, Taiwan
[6] Natl Taiwan Ocean Univ, Ctr Marine Bioenvironment & Biotechnol, Keelung 202301, Taiwan
关键词
chitosan; PLA composite film; EDTA; nisin; antimicrobial activity; fish preservation; ANTIMICROBIAL ACTIVITY; BIODEGRADABLE FILMS; QUALITY; SHRIMP; ACID; OPTIMIZATION; ANTIOXIDANT; EXTRACT; PH;
D O I
10.3390/membranes11110852
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study aimed to increase the antibacterial activity of chitosan-polylactic acid (PLA) composite film by adding nisin and ethylenediaminetetraacetic acid (EDTA). We evaluated the mechanical, physicochemical, and antibacterial properties of various PLA composite films, as well as the enhancement effect of PLA composite films with EDTA + nisin on the preservation of grouper fillets. Films of PLA alone, PLA plus chitosan (C5), PLA plus nisin + EDTA (EN2), and PLA plus chitosan plus nisin + EDTA (C5EN1 and C5EN2) were prepared. The addition of EDTA + nisin to the chitosan-PLA matrix significantly improved the antibacterial activity of the PLA composite film, with C5EN1 and C5EN2 films showing the highest antibacterial activity among the five films. Compared with the fish samples covered by C5, the counts of several microbial categories (i.e., mesophilic bacteria, psychrotrophic bacteria, coliforms, Aeromonas, Pseudomonas, and Vibrio) and total volatile basic nitrogen content in fish were significantly reduced in the samples covered by C5EN1. In addition, the counts of samples covered by C5EN1 or C5 were significantly lower compared to the uncovered and PLA film-covered samples.
引用
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页数:15
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