Use of selected reaction monitoring data for label-free quantification of protein modification stoichiometry

被引：8

作者：

Balasubramaniam, Deepa ^{[2
]}

Eissler, Christie L. ^{[1
]}

Stauffachei, Cynthia V. ^{[2
,3
,4
]}

Hall, Mark C. ^{[1
,3
]}

机构：

[1] Purdue Univ, Dept Biochem, W Lafayette, IN 47907 USA

[2] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

[3] Purdue Univ, Ctr Canc Res, W Lafayette, IN 47907 USA

[4] Purdue Univ, Markey Ctr Struct Biol, W Lafayette, IN 47907 USA

来源：

PROTEOMICS | 2010年 / 10卷 / 23期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

Label-free quantification; MS; PTM; Selected reaction monitoring; Stoichiometry; Technology; MASS-SPECTROMETRY; ABSOLUTE QUANTIFICATION; PHOSPHORYLATION; PROTEOMICS; EXPRESSION; QUANTITATION; KINASE; MS;

D O I：

10.1002/pmic.201000232

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantification of protein and PTM abundance in biological samples is an important component of proteomic studies Label free methods for quantification using MS are attractive because they are simple to implement and applicable to any experimental system We demonstrate that PTM stoichiometry can be accurately measured using label free quantification and selected reaction monitoring Use of selected reaction monitoring is advantageous with complex biological samples and we show this approach can be used to quantify multiple PTMs independently on a single peptide

引用

页码：4301 / 4305

页数：5

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