Shen-Fu Injection Preconditioning Inhibits Myocardial Ischemia-Reperfusion Injury in Diabetic Rats: Activation of eNOS via the PI3K/Akt Pathway

被引：34

作者：

Wu, Yang ^{[1
]}

Xia, Zhong-yuan ^{[1
]}

Meng, Qing-tao ^{[1
]}

Zhu, Jie ^{[2
]}

Lei, Shaoqing ^{[3
]}

Xu, Jinjin ^{[1
]}

Dou, Juan ^{[4
]}

机构：

[1] Wuhan Univ, Renmin Hosp, Dept Anesthesiol, Wuhan 430060, Hubei, Peoples R China

[2] Wuhan Univ, Renmin Hosp, Dept Gastroenterol, Wuhan 430060, Hubei, Peoples R China

[3] Wuhan Univ, Sch Med, Dept Pharmacol, Wuhan 430071, Peoples R China

[4] Wuhan Univ, Renmin Hosp, Dept Cardiovasc Surg, Wuhan 430060, Hubei, Peoples R China

来源：

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2011年

基金：

中国国家自然科学基金;

关键词：

NITRIC-OXIDE SYNTHASE; ISCHEMIA/REPERFUSION INJURY; CARDIOPULMONARY BYPASS; APOPTOSIS; AKT; SURVIVAL; RABBITS; HEART; CARDIOPROTECTION; PHOSPHORYLATION;

D O I：

10.1155/2011/384627

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The aim of this paper is to investigate whether Shen-fu injection (SFI), a traditional Chinese medicine, could attenuate myocardial ischemia-reperfusion (MI/R) injury in diabetes. Streptozotocin-induced diabetic rats were randomly assigned to the Sham, I/R, SFI preconditioning, and SFI plus wortmannin (a phosphatidylinositol 3-kinase inhibitor) groups. After the treatment, hearts were subjected to 30 min of coronary artery occlusion and 2 h reperfusion except the Sham group. Myocardial infarct size and cardiomyocytes apoptosis were increased significantly in MI/R group as compared with the Sham group. SFI preconditioning significantly decreased infarct size, apoptosis, caspase-3 protein expression, MDA level in myocardial tissues, and plasma level of CK and LDH but increased p-Akt, p-eNOS, bcl-2 protein expression, and SOD activity compared to I/R group. Moreover, SFI-induced cardioprotection was abolished by wortmannin. We conclude that SFI preconditioning protects diabetic hearts from I/R injury via PI3K/Akt-dependent pathway.

引用

页数：9

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