Shen-Fu Injection Preconditioning Inhibits Myocardial Ischemia-Reperfusion Injury in Diabetic Rats: Activation of eNOS via the PI3K/Akt Pathway

被引：34
作者：
Wu, Yang [1 ]
Xia, Zhong-yuan [1 ]
Meng, Qing-tao [1 ]
Zhu, Jie [2 ]
Lei, Shaoqing [3 ]
Xu, Jinjin [1 ]
Dou, Juan [4 ]
机构：
[1] Wuhan Univ, Renmin Hosp, Dept Anesthesiol, Wuhan 430060, Hubei, Peoples R China
[2] Wuhan Univ, Renmin Hosp, Dept Gastroenterol, Wuhan 430060, Hubei, Peoples R China
[3] Wuhan Univ, Sch Med, Dept Pharmacol, Wuhan 430071, Peoples R China
[4] Wuhan Univ, Renmin Hosp, Dept Cardiovasc Surg, Wuhan 430060, Hubei, Peoples R China
来源：
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2011年
基金：
中国国家自然科学基金;
关键词：
NITRIC-OXIDE SYNTHASE; ISCHEMIA/REPERFUSION INJURY; CARDIOPULMONARY BYPASS; APOPTOSIS; AKT; SURVIVAL; RABBITS; HEART; CARDIOPROTECTION; PHOSPHORYLATION;
D O I：
10.1155/2011/384627
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The aim of this paper is to investigate whether Shen-fu injection (SFI), a traditional Chinese medicine, could attenuate myocardial ischemia-reperfusion (MI/R) injury in diabetes. Streptozotocin-induced diabetic rats were randomly assigned to the Sham, I/R, SFI preconditioning, and SFI plus wortmannin (a phosphatidylinositol 3-kinase inhibitor) groups. After the treatment, hearts were subjected to 30 min of coronary artery occlusion and 2 h reperfusion except the Sham group. Myocardial infarct size and cardiomyocytes apoptosis were increased significantly in MI/R group as compared with the Sham group. SFI preconditioning significantly decreased infarct size, apoptosis, caspase-3 protein expression, MDA level in myocardial tissues, and plasma level of CK and LDH but increased p-Akt, p-eNOS, bcl-2 protein expression, and SOD activity compared to I/R group. Moreover, SFI-induced cardioprotection was abolished by wortmannin. We conclude that SFI preconditioning protects diabetic hearts from I/R injury via PI3K/Akt-dependent pathway.
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页数：9
相关论文
共 39 条
[1]   Revascularization for coronary artery disease in diabetes mellitus: Angioplasty, stents and coronary artery bypass grafting [J].
Aronson, Doron ;
Edelman, Elazer R. .
REVIEWS IN ENDOCRINE & METABOLIC DISORDERS, 2010, 11 (01) :75-86
[2]   Diabetic cardiomyopathy revisited [J].
Boudina, Sihem ;
Abel, E. Dale .
CIRCULATION, 2007, 115 (25) :3213-3223
[3]   Nitric oxide and cell signaling pathways in mitochondrial-dependent apoptosis [J].
Boyd, CS ;
Cadenas, E .
BIOLOGICAL CHEMISTRY, 2002, 383 (3-4) :411-423
[4]   PPAR-α activation protects the type 2 diabetic myocardium against ischemia-reperfusion injury: involvement of the PI3-Kinase/Akt and NO pathway [J].
Bulhak, Aliaksandr A. ;
Jung, Christian ;
Ostenson, Claes-Goran ;
Lundberg, Jon O. ;
Sjoquist, Per-Ove ;
Pernow, John .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2009, 296 (03) :H719-H727
[5]   Phosphodiesterase-5 inhibitor sildenafil preconditions adult cardiac myocytes against necrosis and apoptosis [J].
Das, A ;
Xi, L ;
Kukreja, RC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (13) :12944-12955
[6]   Diabetes and mortality following acute coronary syndromes [J].
Donahoe, Sean M. ;
Stewart, Garrick C. ;
McCabe, Carolyn H. ;
Mohanavelu, Satishkumar ;
Murphy, Sabina A. ;
Cannon, Christopher P. ;
Antman, Elliott M. .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2007, 298 (07) :765-775
[7]   Mammalian caspases: Structure, activation, substrates, and functions during apoptosis [J].
Earnshaw, WC ;
Martins, LM ;
Kaufmann, SH .
ANNUAL REVIEW OF BIOCHEMISTRY, 1999, 68 :383-424
[8]   Molecular indices of apoptosis after intermittent blood and crystalloid cardioplegia [J].
Feng, J ;
Bianchi, C ;
Sandmeyer, JL ;
Li, JY ;
Sellke, FW .
CIRCULATION, 2005, 112 (09) :I184-I189
[9]   Nitric oxide mediates the antiapoptotic effect of insulin in myocardial ischemia-reperfusion -: The roles of PI3-kinase, akt, and endothelial nitric oxide synthase phosphorylation [J].
Gao, F ;
Gao, E ;
Yue, TL ;
Ohlstein, EH ;
Lopez, BL ;
Christopher, TA ;
Ma, XL .
CIRCULATION, 2002, 105 (12) :1497-1502
[10]   Egr-1, a Central and Unifying Role in Cardioprotection from Ischemia-Reperfusion Injury? [J].
Gao, Fen-Fei ;
Jia, Qiang-Yong ;
Guo, Fu-Xiao ;
Zhang, Yan-Mei ;
Huang, Zhan-Qin ;
Zhou, Yan-Qiong ;
Chen, Yi-Cun ;
Shi, Gang-Gang .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2009, 24 (5-6) :519-526
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