A SENSITIVE SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DIOSMIN USING SODIUM NITROPRUSSIDE AS A CHROMOGENIC REAGENT

被引：12
|
作者：
Moldovan, Zenovia [1 ]
Aboul-Enein, Hassan Y. [2 ]
机构：
[1] Univ Bucharest, Dept Analyt Chem, Bucharest, Romania
[2] Natl Res Ctr, Pharmaceut & Med Chem Dept, Cairo 12311, Egypt
来源：
INSTRUMENTATION SCIENCE & TECHNOLOGY | 2011年 / 39卷 / 02期
关键词：
Diosmin; hydroxylamine hydrochloride; sodium nitroprusside; spectrophotometry; TRACE AMOUNTS; HESPERIDIN; PHENOL; WATER; CARCINOGENESIS;
D O I：
10.1080/10739149.2010.545851
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple and sensitive method for the spectrophotometric determination of diosmin has been developed, and optimum reaction conditions along with other analytical parameters have been evaluated. Diosmin, as a phenolic compound, reacts with sodium nitroprusside and hydroxylamine hydrochloride in a basic medium to give a green-colored product. At analytical wavelength of 763nm, Beer's law is obeyed over the concentration range 2-140gmL-1 of diosmin with a correlation coefficient of 0.9997. The molar absorptivity is 9671L mol-1cm-1. The limits of detection (LOD) and quantification (LOQ) are 0.05 and 0.2.gmL-1, respectively. The method has been successfully applied to the determination of diosmin in a commercial pharmaceutical preparation. The results were compared favorably with those obtained by the 4-aminoantipyrine method.
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页码：135 / 148
页数：14
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