Prevalence of serum antibodies to Mycobacterium avium subsp paratuberculosis in cattle in Galicia (northwest Spain)

被引:18
作者
Dieguez, Francisco J.
Arnaiz, Ignacio
Sanjuan, Maria L.
Vilar, Maria J.
Lopez, Manuel
Yus, Eduardo
机构
[1] Univ Santiago Compostela, Inst Food Anal & Res, Epidemiol & Anim Hlth Unit, Lugo 27002, Spain
[2] Anim Hlth & Prod Lab Galicia, Lugo 27002, Spain
关键词
cattle; Johne's disease; prevalence;
D O I
10.1016/j.prevetmed.2007.08.006
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Prior to establishing a control and prevention program for Johne's disease in cattle in Galicia (northwest Spain), a survey was conducted to estimate the prevalence of the disease. For this survey, 61,069 animals of at least 1-year of age from 2735 randomly selected herds were bled and samples analyzed with a commercial ELISA. The estimated true individual-level prevalences - assuming the manufacturer's reported test sensitivity of 48.5% and specificity of 98.9% - were 3.02% in dairy cattle, 1.03% in beef cattle and 2.83% in animals from farms with both dairy and beef cattle. True herd prevalences (with herds declared positive if one or more animals tested positive) were 10.69% for dairy herds, 0% for beef herds and 2.7 1 % for mixed herds. When herds were declared positive if at least two animals tested positive, true herd prevalences were 14.75% for dairy herds, 1.47% for beef herds and 12.01 % for mixed herds. Assuming a higher specificity of 99.4%, true individual-level prevalences increased to 4.03% in dairy herds, 2.07% in beef herds and 3.84% in mixed herds. Herd prevalences were 27.77%/18.79%, 2.78%/2.40% and 5.70%/ 12.24% (using the one/two-animal cut-offs) in dairy, beef and mixed herds, respectively. In conclusion, these results seem to indicate that a small percentage of cows and a rather high percentage of dairy herds in this region are MAP-seropositive. (c) 2007'Elsevier B.V. All rights reserved.
引用
收藏
页码:321 / 326
页数:6
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