Accelerated ex situ breeding of GBSS- and PTST1-edited cassava for modified starch

被引:115
作者
Bull, Simon E. [1 ,4 ]
Seung, David [1 ,2 ,5 ]
Chanez, Christelle [1 ]
Mehta, Devang [1 ]
Kuon, Joel-Elias [1 ]
Truernit, Elisabeth [1 ,2 ]
Hochmuth, Anton [1 ,2 ]
Zurkirchen, Irene [1 ]
Zeeman, Samuel C. [1 ,2 ]
Gruissem, Wilhelm [1 ]
Vanderschuren, Herve [1 ,3 ]
机构
[1] Swiss Fed Inst Technol, Inst Mol Plant Biol, Plant Biotechnol, CH-8092 Zurich, Switzerland
[2] Swiss Fed Inst Technol, Inst Mol Plant Biol, Plant Biochem, CH-8092 Zurich, Switzerland
[3] Univ Liege, Plant Genet, TERRA Teaching & Res Ctr, Gembloux Agrobio Tech, B-5030 Gembloux, Belgium
[4] Swiss Fed Inst Technol, Mol Plant Breeding, Inst Agr Sci, CH-8092 Zurich, Switzerland
[5] John Innes Ctr, Norwich NR4 7UH, Norfolk, England
来源
SCIENCE ADVANCES | 2018年 / 4卷 / 09期
关键词
AMYLOSE-FREE STARCH; GENE-EXPRESSION; PASTING PROPERTIES; ARABIDOPSIS; CROP; RNA; WAXY; TOOL; DNA; GELATINIZATION;
D O I
10.1126/sciadv.aat6086
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Crop diversification required to meet demands for food security and industrial use is often challenged by breeding time and amenability of varieties to genome modification. Cassava is one such crop. Grown for its large starch-rich storage roots, it serves as a staple food and a commodity in the multibillion-dollar starch industry. Starch is composed of the glucose polymers amylopectin and amylose, with the latter strongly influencing the physicochemical properties of starch during cooking and processing. We demonstrate that CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)-mediated targeted mutagenesis of two genes involved in amylose biosynthesis, PROTEIN TARGETING TO STARCH (PTST1) or GRANULE BOUND STARCH SYNTHASE (GBSS), can reduce or eliminate amylose content in root starch. Integration of the Arabidopsis FLOWERING LOCUS T gene in the genome-editing cassette allowed us to accelerate flowering-an event seldom seen under glasshouse conditions. Germinated seeds yielded S1, a transgene-free progeny that inherited edited genes. This attractive new plant breeding technique for modified cassava could be extended to other crops to provide a suite of novel varieties with useful traits for food and industrial applications.
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页数:12
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