Relaxant Action of Plumula Nelumbinis Extract on Mouse Airway Smooth Muscle

被引：12

作者：

Tan, Li ^{[1
,2
]}

Chen, Weiwei ^{[1
,2
]}

Wei, Ming-Yu ^{[1
,2
]}

Shen, Jinhua ^{[1
,2
]}

Yu, Meng-Fei ^{[1
,2
]}

Yang, Guangzhong ^{[3
]}

Guo, Donglin ^{[4
,5
]}

Qin, Gangjian ^{[6
]}

Ji, Guangju ^{[7
]}

Liu, Qing-Hua ^{[1
,2
]}

机构：

[1] South Cent Univ Nationalities, Inst Med Biol, Coll Life Sci, Wuhan 430074, Peoples R China

[2] South Cent Univ Nationalities, Hubei Prov Key Lab Protect & Applicat Special Pla, Coll Life Sci, Wuhan 430074, Peoples R China

[3] South Cent Univ Nationalities, Coll Pharm, Wuhan 430074, Peoples R China

[4] Lankenau Inst Med Res, Wynnewood, PA 19096 USA

[5] Main Line Hlth Heart Ctr, Wynnewood, PA 19096 USA

[6] Northwestern Univ, Feinberg Sch Med, Dept Med Cardiol, Feinberg Cardiovasc Res Inst, Chicago, IL 60611 USA

[7] Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2015年 / 2015卷

基金：

中国国家自然科学基金;

关键词：

CA2+ RELEASE; CALCIUM; CELLS; ACTIVATION; ASTHMA; PROLIFERATION; CONTRACTILITY; CHANNELS; NEFERINE; STORE;

D O I：

10.1155/2015/523640

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

The traditional herb Plumula Nelumbinis is widely used in the world because it has many biological activities, such as anti-inflammation, antioxidant, antihypertension, and butyrylcholinesterase inhibition. However, the action of Plumula Nelumbinis on airway smooth muscle (ASM) relaxation has not been investigated. A chloroform extract of Plumula Nelumbinis (CEPN) was prepared, which completely inhibited precontraction induced by high K+ in a concentration-dependent manner in mouse tracheal rings, but it had no effect on resting tension. CEPN also blocked voltage-dependent L-type Ca2+ channel-(VDCC-) mediated currents. In addition, ACh-induced precontraction was also completely blocked by CEPN and partially inhibited by nifedipine or pyrazole 3. Besides, CEPN partially reduced ACh-activated nonselective cation channel (NSCC) currents. Taken together, our data demonstrate that CEPN blocked VDCC and NSCC to inhibit Ca2+ influx, resulting in relaxation of precontracted ASM. This finding indicates that CEPN would be a candidate of new potent bronchodilators.

引用

页数：10

共 50 条

[31] Relaxing Effect of TSU-68, an Antiangiogenic Agent, on Mouse Airway Smooth Muscle [J].

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Lei, Jun ;

Xue, Lu ;

Cai, Congli ;

Liu, Qing-hua ;

Shen, Jinhua .

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2017, 41 (06) :2350-2362

[32] Role of airway smooth muscle in inflammation [J].

Frossard, N .

REVUE DES MALADIES RESPIRATOIRES, 2000, 17 (2BIS) :559-563

[33] Airway smooth muscle [J].

Stephens, NL .

LUNG, 2001, 179 (06) :333-373

[34] Airway smooth muscle proliferation and survival is not modulated by mast cells [J].

Kaur, D. ;

Hollins, F. ;

Saunders, R. ;

Woodman, L. ;

Sutcliffe, A. ;

Cruse, G. ;

Bradding, P. ;

Brightling, C. .

CLINICAL AND EXPERIMENTAL ALLERGY, 2010, 40 (02) :279-288

[35] Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle [J].

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Luo, Xiao-Jing ;

Sai, Wen-Bo ;

Yu, Meng-Fei ;

Li, Wen-Er ;

Ma, Yun-Fei ;

Chen, Weiwei ;

Zhai, Kui ;

Qin, Gangjian ;

Guo, Donglin ;

Zheng, Yun-Min ;

Wang, Yong-Xiao ;

Shen, Jin-Hua ;

Ji, Guangju ;

Liu, Qing-Hua .

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[36] TNFα decreases mitochondrial movement in human airway smooth muscle [J].

Delmotte, Philippe ;

Zavaletta, Vanessa A. ;

Thompson, Michael A. ;

Prakash, Y. S. ;

Sieck, Gary C. .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2017, 313 (01) :L166-L176

[37] Airway smooth muscle chemokine receptor expression and function in asthma [J].

Saunders, R. ;

Sutcliffe, A. ;

Kaur, D. ;

Siddiqui, S. ;

Hollins, F. ;

Wardlaw, A. ;

Bradding, P. ;

Brightling, C. .

CLINICAL AND EXPERIMENTAL ALLERGY, 2009, 39 (11) :1684-1692

[38] Effects of cigarette smoke extract on human airway smooth muscle cells in COPD [J].

Chen, Ling ;

Ge, Qi ;

Tjin, Gavin ;

Alkhouri, Hatem ;

Deng, Linghong ;

Brandsma, Corry-Anke ;

Adcock, Ian ;

Timens, Wim ;

Postma, Dirkje ;

Burgess, Janette K. ;

Black, Judith L. ;

Oliver, Brian G. G. .

EUROPEAN RESPIRATORY JOURNAL, 2014, 44 (03) :634-646

[39] Bitter taste receptors on airway smooth muscle bronchodilate by localized calcium signaling and reverse obstruction [J].

Deshpande, Deepak A. ;

Wang, Wayne C. H. ;

McIlmoyle, Elizabeth L. ;

Robinett, Kathryn S. ;

Schillinger, Rachel M. ;

An, Steven S. ;

Sham, James S. K. ;

Liggett, Stephen B. .

NATURE MEDICINE, 2010, 16 (11) :1299-U8

[40] Isolation and identification of a tribenzylisoquinoline alkaloid from Nelumbo nucifera Gaertn, a novel potential smooth muscle relaxant [J].

Yang, Guang-Ming ;

Sun, Jie ;

Pan, Yang ;

Zhang, Jun-Li ;

Xiao, Min ;

Zhu, Min-Sheng .

FITOTERAPIA, 2018, 124 :58-65

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