Rapid and automated on-line solid phase extraction HPLC-MS/MS with peak focusing for the determination of ochratoxin A in wine samples

被引：84

作者：

Campone, Luca ^{[1
,2
]}

Piccinelli, Anna Lisa ^{[1
]}

Celano, Rita ^{[1
]}

Pagano, Imma ^{[1
]}

Russo, Mariateresa ^{[2
]}

Rastrelli, Luca ^{[1
]}

机构：

[1] Univ Salerno, Dipartimento Farm, Via Giovanni Paolo 2, I-84084 Fisciano, SA, Italy

[2] Univ Mediterranea Reggio Calabria, Dipartimento Agr, I-89122 Reggio Di Calabria, RC, Italy

来源：

FOOD CHEMISTRY | 2018年 / 244卷

关键词：

Ochratoxin A; On-line SPE; HPLC-MS/MS; Red wine and white wine; Food safety; Oasis MAX; TANDEM MASS-SPECTROMETRY; LIQUID-LIQUID MICROEXTRACTION; CHROMATOGRAPHY; AFLATOXINS; CLEANUP; BEER; PRODUCTS;

D O I：

10.1016/j.foodchem.2017.10.023

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study reports a fast and automated analytical procedure based on an on-line SPE-HPLC-MS/MS method for the automatic pre-concentration, clean up and sensitive determination of OTA in wine. The amount of OTA contained in 100 mu L of sample (pH. 5.5) was retained and concentrated on an Oasis MAX SPE cartridge. After a washing step to remove matrix interferents, the analyte was eluted in back-flush mode and the eluent from the SPE column was diluted through a mixing Tee, using an aqueous solution before the chromatographic separation achieved on a monolithic column. The developed method has been validated according to EU regulation N. 519/2014 and applied for the analysis of 41 red and 17 white wines. The developed method features minimal sample handling, low solvent consumption, high sample throughput, low analysis cost and provides an accurate and highly selective results.

引用

收藏

页码：128 / 135

页数：8

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