Spectrophotometric determination of total proteins by derivatization with p-benzoquinone in a flow system

被引：2

作者：

Lichtig, J

Guarilha, O

Oliveira, RMS

Masini, JC

Trujillo, LM

机构：

[1] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo, Brazil

[2] Inst Adolfo Lutz, BR-01246902 Sao Paulo, Brazil

来源：

ANALYTICAL LETTERS | 2001年 / 34卷 / 02期

关键词：

p-benzoquinone; total proteins; derivatization; flow system; reactor; spectrophotometric assay;

D O I：

10.1081/AL-100001571

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The determination of total proteins in plasma is largely needed in clinical and biochemical areas because it is important for the diagnosis of several diseases. This paper describes a flow method for determination of total protein in human serum albumin samples using p-benzoquinone (PBQ) dissolved in dimethyl-sulphoxide (DMSO) as reagent. The reactional mixture at pH 8.0 or 9.0 was pumped through a coiled stainless steel capillary tube reactor, heated at 90 degreesC, to a spectrophotometric flow cell where the absorbance was monitored at 361 nm. A linear dynamic range was observed for protein concentrations from 50 to 500 mug mL(-1). Results were in good agreement with those obtained by the Kjeldahl's method.

引用

页码：193 / 200

页数：8

共 22 条

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