Oxacillin-resistant coagulase-negative Staphylococci bacteremia at a teaching hospital in Santa Maria, State of Rio Grande do Sul, Brazil

被引：5

作者：

Rigatti, Fabiane ^{[1
]}

Tizotti, Maisa Kraulich ^{[1
]}

Hoerner, Rosmari ^{[2
]}

Domingues, Vanessa Oliveira

Martini, Rosieli ^{[1
]}

Mayer, Leticia Eichstaedt ^{[1
]}

Khun, Fabio Teixeira ^{[3
]}

de Franca, Chirles Araujo ^{[4
]}

da Costa, Mateus Matiuzzi ^{[4
]}

机构：

[1] Univ Fed Santa Maria, Curso Posgrad Ciencias Farmaceut, BR-97119900 Santa Maria, RS, Brazil

[2] Univ Fed Santa Maria, Dept Anal Clin & Toxicol, BR-97119900 Santa Maria, RS, Brazil

[3] Univ Fed Santa Maria, Curso Farm, BR-97119900 Santa Maria, RS, Brazil

[4] Univ Fed Vale do Sao Francisco, Curso Pos Grad Ciencia Anim, Petrolina, PE, Brazil

来源：

REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL | 2010年 / 43卷 / 06期

关键词：

Bacteremia; Nosocomial infection; Coagulase-negative staphylococci; mecA gene; Cefoxitin; BLOOD-STREAM INFECTIONS; INTENSIVE-CARE-UNITS; GRAM-POSITIVE COCCI; METHICILLIN RESISTANCE; NOSOCOMIAL INFECTIONS; IDENTIFICATION; SURVEILLANCE; HAEMOLYTICUS; EPIDERMIDIS; DAPTOMYCIN;

D O I：

10.1590/S0037-86822010000600017

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Introduction: This study aimed to characterize the prevalence and susceptibility profile to oxacillin-resistant Coagulase-negative Staphylococci strains isolated from blood cultures in a teaching hospital, located in Santa Maria, RS. In addition, different methodologies for phenotypic characterization of mecA-mediated oxacillin resistance were compared with genotypic reference testing. Methods: After identification (MicroScan (R) - Siemens), the isolates were tested for antimicrobial sensitivity using disk diffusion and automation (MicroScan (R) - Siemens). The presence of mecA gene was identified by the polymerase chain reaction molecular technique. Results: The most common species was Staphylococcus epidermidis (n=40, 67%). The mecA gene was detected in 54 (90%) strains, while analysis of the sensitivity profiles revealed a high rate of resistance to multiple classes of antimicrobial drugs. However, all isolates were uniformly sensitive to vancomycin and tigecycline. The cefoxitin disk was the phenotypic method that best correlated with the gold standard. Conclusions: Analysis of the clinical significance of CoNS isolated from hemocultures and the precise detection of oxacillin resistance represent decisive factors for the correct choice of antibiotic therapy. Although vancomycin constitutes the normal treatment in most Brazilian hospitals, reduction in its use is recommended.

引用

页码：686 / 690

页数：5