Vitamin D supplement improved testicular function in diabetic rats

被引：37

作者：

Ding, Chenzhao ^{[1
,2
]}

Wang, Qinzhu ^{[1
]}

Hao, Yue ^{[1
,2
]}

Ma, Xiaojun ^{[1
,2
]}

Wu, Lina ^{[1
,2
]}

Du, Mengmeng ^{[1
,2
]}

Li, Wen ^{[1
,2
]}

Wu, Yang ^{[1
,2
]}

Guo, Feng ^{[1
,2
]}

Ma, Siyuan ^{[1
,2
]}

Huang, Fengjuan ^{[1
,2
]}

Qin, Guijun ^{[1
]}

机构：

[1] Zhengzhou Univ, Affiliated Hosp 1, Dept Internal Med, Div Endocrinol, 40 Daxue Rd, Zhengzhou 450052, Peoples R China

[2] Zhengzhou Univ, Affiliated Hosp 1, Inst Clin Med, Zhengzhou 450052, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 473卷 / 01期

关键词：

Diabetes; Testicular damage; 1,25(OH)2D3; Inflammation; Apoptosis; OXIDATIVE STRESS; 1-ALPHA; 25-DIHYDROXYVITAMIN D-3; HYPERGLYCEMIA; EXPRESSION; APOPTOSIS; COMPLICATIONS; FERTILITY; PROTEINS; PATHWAY; SYSTEM;

D O I：

10.1016/j.bbrc.2016.03.072

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was designed to investigate the role that 1,25(OH)2D3 plays against testicular lesion in diabetic rats and try to find its possible mechanism of the steroidogenesis and the spermatogenesis. In diabetic rats, prolonged hyperglycemia evaluated inflammatory cytokines, damaged sperm production function and redox balance, diminished serum testosterone. After treated with 1,25(OH)2D3 at two different doses respectively for 12 months, all the alternations were effectively normalized. 1,25(OH)2D3 showed inhibitory effect on excessive inflammatory biomarkers and adjusted the expression reproductive genes and testicular androgen synthesis. It also upregulated Bcl-2 expression, decreased Bax and COX -2 expression and inhibited active caspase cascade (caspase 8 and caspase 3), which may preserved the testicular cells under diabetic condition. It revealed that vitamin D supplement may protect the cells through suppressing inflammation factors and alleviating cell apoptotic death, as well as upregulating the expression of genes related to reproductive and testosterone synthesis. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：161 / 167

页数：7

共 33 条

[1] Relative expression of 1,25-dihydroxyvitamin D3 receptor, vitamin D 1α-hydroxylase, vitamin D 24-hydroxylase, and vitamin D 25-hydroxylase in endometriosis and gynecologic cancers [J].