Deregulation of the Sod1 and Nd1 genes in mouse fetal oocytes exposed to mono-(2-ethylhexyl) phthalate (MEHP)

被引：44

作者：

Bonilla, Edmundo ^{[1
,2
]}

del Mazo, Jesus ^{[1
]}

机构：

[1] CSIC, Ctr Invest Biol, Dept Cell Proliferat & Dev, Madrid 28040, Spain

[2] Univ Autonoma Metropolitana Iztapalapa, Dept Ciencias Salud, Mexico City 09340, DF, Mexico

来源：

REPRODUCTIVE TOXICOLOGY | 2010年 / 30卷 / 03期

关键词：

Fetal oocytes; Gene expression; MEHP; mono-(2-ethylhexyl); phthalate; Mitochondrial respiratory chain; Nd1; NADH-ubiquinone oxidoreductase chain 1; Reproductive toxicology; Sod1; Cu-Zn superoxide dismutase; RAT GRANULOSA-CELLS; OXIDATIVE STRESS; IN-UTERO; DI(2-ETHYLHEXYL) PHTHALATE; DI(N-BUTYL) PHTHALATE; SUPPRESSES ESTRADIOL; LACTATIONAL EXPOSURE; HUMAN-REPRODUCTION; CYCLING RATS; DEHP;

D O I：

10.1016/j.reprotox.2010.04.008

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mono-(2-ethylhexyl) phthalate (MEHP) is the active metabolite of the diester (DEHP), a well-known reproductive toxicant. Since, different molecular mechanisms underlying this toxicity are not well understood, the effects of MEHP on cell viability and gene expression were assessed in murine fetal oocytes cultured in vitro. Oocyte survival decreased significantly after a 24 h exposure to MEHP and hence, the effects of MEHP on changes in gene expression were analyzed using cDNA libraries and differential screenings. In these assays, the genes that suffered the most severe deregulation corresponded to those encoding Cu-Zn superoxide dismutase (Sod1) and a mitochondrial respiratory chain protein (Nd1). Indeed, functional assays on somatic cells transfected with a Sod1 luciferase reporter construct demonstrated its specific MEHP dose-dependent up-regulation, confirming that the expression of this gene is deregulated in response to MEHP. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：387 / 392

页数：6

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