Rapid identification of virulent type I strains of the protozoan pathogen Toxoplasma gondii by PCR-restriction fragment length polymorphism analysis at the B1 gene

被引：142

作者：

Grigg, ME ^{[1
]}

Boothroyd, JC ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 01期

关键词：

D O I：

10.1128/JCM.39.1.398-400.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sequence analysis at the 35-fold-repetitive B1 locus identified three restriction sites capable of discriminating type I (mouse-virulent) from type II or III (mouse-avirulent) strains of Toxoplasma gondii. B1 PCR-restriction fragment length polymorphism analysis of 8 type I, 17 type II, and 8 type III strains confirms the specificity of the assay. It should now be possible to ask whether strain genotype affects the severity and type of clinical disease in humans.

引用

页码：398 / 400

页数：3

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