Differential diagnosis of HIV type 1 group O and M infection by polymerase chain reaction and PstI restriction analysis of the pol gene fragment

被引：12

作者：

Heyndrickx, L

Janssens, W

Gürtler, L

Zekeng, L

Loussert-Ajaka, I

Vereecken, K

Willems, B

Coppens, S

Ndumbe, P

Fransen, K

Saman, E

Alary, M

Van der Groen, G

机构：

[1] Inst Trop Med, Dept Microbiol, B-2000 Antwerp, Belgium

[2] Univ Munich, D-80336 Munich, Germany

[3] Ctr Hosp Univ, Yaounde, Cameroon

[4] Hop Bichat Claude Bernard, F-75877 Paris, France

[5] Ctr Study & Control Communicable Dis, Yaounde, Cameroon

[6] Innogenet NV, B-9052 Ghent, Belgium

[7] Hop St Sacrement, Ctr Rech, Quebec City, PQ G1S 4L8, Canada

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 1998年 / 14卷 / 11期

关键词：

D O I：

10.1089/aid.1998.14.973

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

HIV-1 group O serological screening or confirmation strategies so far have not proved 100% sensitive and specific, indicating a lack of antibody reactivity or cross-reactivity with group O antigens, Therefore, genetic analysis currently represents the only method by which confirm presumed HIV-1 group O or group O/M infections. We have optimized the sensitivity (100%) and specificity (100%) of an HIV-1 group O/M-specific PCR of a pol gene fragment. In addition, me report on a highly sensitive (97.2%) and specific (100%) method for differentiation between HIV-1 group O and group M viruses, using PCR and PstI enzyme restriction fragment analysis of a pol fragment, Compared with sequencing, these methods are fast, inexpensive, and simple.

引用

页码：973 / 977

页数：5

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