Functional Expression of phaCAB Genes from Cupriavidus taiwanensis Strain 184 in Escherichia coli for Polyhydroxybutyrate Production

被引：13
|
作者：
Chien, Chih-Ching [1 ]
Hong, Chian-Chiao [1 ]
Soo, Po-Chi [3 ]
Wei, Yu-Hong [1 ]
Chen, Shan-Yu [1 ]
Cheng, Mei-Ling [2 ]
Sun, Yi-Ming [2 ]
机构：
[1] Yuan Ze Univ, Grad Sch Biotechnol & Bioengn, Chungli 320, Taiwan
[2] Yuan Ze Univ, Dept Chem Engn & Mat Sci, Chungli 320, Taiwan
[3] Tzu Chi Univ, Dept Lab Med & Biotechnol, Hualien 970, Taiwan
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2010年 / 162卷 / 08期
关键词：
Polyhydroxyalkanoates; PHA; Cupriavidus taiwanensis; PHB; Polyhydroxybutyrate; POLY-BETA-HYDROXYBUTYRATE; POLYHYDROXYALKANOATES; BIOSYNTHESIS; POLYESTER; BACTERIA; ACID; PHB;
D O I：
10.1007/s12010-010-9008-y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Polyhydroxyalkanoates are polyesters synthesized by numerous microorganisms. These polyesters are biodegradable and have similar properties to those of conventional plastics. Cupriavidus taiwanensis strain 184 is phylogenetically related to the well-known polyhydroxybutyrate (PHB) producer Ralstonia eutropha (Cupriavidus necator) and is also shown to be able to accumulate significant amounts of PHB. In this study, we cloned the PHB synthesis genes (phaCAB) from C. taiwanensis 184 into Escherichia coli for biosynthesis of PHB. The recombinant E. coli strains were able to synthesize significant amounts of PHB. The PHB amounted to about 66 similar to 70% of total cell material of these recombinant strains.
引用
收藏
页码：2355 / 2364
页数：10
相关论文
共 50 条
  • [1] Functional Expression of phaCAB Genes from Cupriavidus taiwanensis Strain 184 in Escherichia coli for Polyhydroxybutyrate Production
    Chih-Ching Chien
    Chian-Chiao Hong
    Po-Chi Soo
    Yu-Hong Wei
    Shan-Yu Chen
    Mei-Ling Cheng
    Yi-Ming Sun
    Applied Biochemistry and Biotechnology, 2010, 162 : 2355 - 2364
  • [2] Functional cis-expression of phaCAB genes for poly(3-hydroxybutyrate) production by Escherichia coli
    Horng, Y. -T.
    Chien, C. -C.
    Wei, Y. -H.
    Chen, S. -Y.
    Lan, J. C. -W.
    Sun, Y. -M.
    Soo, P. -C.
    LETTERS IN APPLIED MICROBIOLOGY, 2011, 52 (05) : 475 - 483
  • [3] Cloning of phaCAB genes from thermophilic Caldimonas manganoxidans in Escherichia coli for poly(3-hydroxybutyrate) (PHB) production
    Lin, Ji-Hong
    Lee, Ming-Chieh
    Sue, You-Sheng
    Liu, Yung-Chuan
    Li, Si-Yu
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2017, 101 (16) : 6419 - 6430
  • [4] Enhanced polyhydroxybutyrate (PHB) production via the coexpressed phaCAB and vgb genes controlled by arabinose PBAD promoter in Escherichia coli
    Horng, Y-T.
    Chang, K-C.
    Chien, C-C.
    Wei, Y-H.
    Sun, Y-M.
    Soo, P-C.
    LETTERS IN APPLIED MICROBIOLOGY, 2010, 50 (02) : 158 - 167
  • [5] Efficient production of polyhydroxybutyrate from slaughterhouse waste using a recombinant strain of Cupriavidus necator DSM 545
    Rodriguez, Jesus E.
    Brojanigo, Silvia
    Basaglia, Marina
    Favaro, Lorenzo
    Casella, Sergio
    SCIENCE OF THE TOTAL ENVIRONMENT, 2021, 794
  • [6] Polyhydroxyalkanoate production from sucrose by Cupriavidus necator strains harboring csc genes from Escherichia coli W
    Arikawa, Hisashi
    Matsumoto, Keiji
    Fujiki, Tetsuya
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2017, 101 (20) : 7497 - 7507
  • [7] Production of blue-colored polyhydroxybutyrate (PHB) by one-pot production and coextraction of indigo and PHB from recombinant Escherichia coli
    Jung, Hye-Rim
    Choi, Tae-Rim
    Han, Yeong Hoon
    Park, Ye-Lim
    Park, Jun Young
    Song, Hun-Suk
    Yang, Soo-Yeon
    Bhatia, Shashi Kant
    Gurav, Ranjit
    Park, HyunA
    Namgung, Seyun
    Kwon-Young, Choi
    Yang, Yung-Hun
    DYES AND PIGMENTS, 2020, 173
  • [8] Use of agro-industrial residue from the canned pineapple industry for polyhydroxybutyrate production by Cupriavidus necator strain A-04
    Sukruansuwan, Vibhavee
    Napathorn, Suchada Chanprateep
    BIOTECHNOLOGY FOR BIOFUELS, 2018, 11
  • [9] Polyhydroxyalkanoate production from sucrose by Cupriavidus necator strains harboring csc genes from Escherichia coli W
    Hisashi Arikawa
    Keiji Matsumoto
    Tetsuya Fujiki
    Applied Microbiology and Biotechnology, 2017, 101 : 7497 - 7507
  • [10] Polyhydroxybutyrate production by recombinant Escherichia coli based on genes related to synthesis pathway of PHB from Massilia sp. UMI-21
    Jiang, Nan
    Wang, Ming
    Song, Linxin
    Yu, Dengbin
    Zhou, Shuangzi
    Li, Yu
    Li, Haiyan
    Han, Xuerong
    MICROBIAL CELL FACTORIES, 2023, 22 (01)
12345