Screening and Evaluation of Human Intestinal Lactobacilli for the Development of Novel Gastrointestinal Probiotics

被引：39

作者：

Koll, Piret ^{[1
]}

Maendar, Reet ^{[1
]}

Smidt, Imbi ^{[1
]}

Huett, Pirje ^{[1
]}

Truusalu, Kai ^{[1
]}

Mikelsaar, Raik-Hiio ^{[1
]}

Shchepetova, Jelena ^{[1
]}

Krogh-Andersen, Kasper ^{[2
]}

Marcotte, Harold ^{[2
]}

Hammarstrom, Lennart ^{[2
]}

Mikelsaar, Marika ^{[1
]}

机构：

[1] Univ Tartu, Dept Microbiol, EE-50090 Tartu, Estonia

[2] Karolinska Inst, Karolinska Univ Hosp Huddinge, Div Clin Immunol, Dept Lab Med, Stockholm, Sweden

来源：

CURRENT MICROBIOLOGY | 2010年 / 61卷 / 06期

关键词：

RESISTANCE DETERMINANT; ORAL LACTOBACILLI; CHAIN ANTIBODY; SAFETY; SUSCEPTIBILITY; FRAGMENTS; BACTERIA; DELIVERY; STRAINS; SPP;

D O I：

10.1007/s00284-010-9653-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aim of this study was to screen intestinal lactobacilli strains for their advantageous properties to select those that could be used for the development of novel gastrointestinal probiotics. Ninety-three isolates were subjected to screening procedures. Fifty-nine percent of the examined lactobacilli showed the ability to auto-aggregate, 97% tolerated a high concentration of bile (2% w/v), 50% survived for 4 h at pH 3.0, and all strains were unaffected by a high concentration of pancreatin (0.5% w/v). One Lactobacillus buchneri strain was resistant to tetracycline. None of the tested strains caused lysis of human erythrocytes. Six potential probiotic strains were selected for safety evaluation in a mouse model. Five of 6 strains caused no translocation, and were considered safe. In conclusion, several strains belonging to different species and fermentation groups were found that have properties required for a potential probiotic strain. This study was the first phase of a multi-phase study aimed to develop a novel, safe and efficient prophylactic and therapeutic treatment system against gastrointestinal infections using genetically modified probiotic lactobacilli.

引用

页码：560 / 566

页数：7

共 34 条

[1] Characterization of intestinal lactobacilli as putative probiotic candidates [J].