Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD

被引：34
作者：
Bastianel, M
Schwarz, SF
Della Coleta, H
Lin, LL
Machado, M
Koller, OC
机构：
[1] Univ Fed Rio Grande Sul, Fac Agron, Dept Hort & Silivicultura, BR-91501970 Porto Alegre, RS, Brazil
[2] IAC, Ctr Citricultura Sylvio Moreira, Cordeiropolis, SP, Brazil
[3] UFSCAR, Ctr Ciencias Agr, Araras, SP, Brazil
来源：
GENETICS AND MOLECULAR BIOLOGY | 1998年 / 21卷 / 01期
关键词：
D O I：
10.1590/S1415-47571998000100020
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The randomly amplified polymorphic DNA (RAPD) technique was used to distinguish nucellar and zygotic seedlings resulting from crosses between the 'Montenegrina' (Citrus deliciosa Tenore) and 'King' (C. nobilis Loureiro) tangerines. The aim of the present study was to develop tangerine varieties with a reduced number of seeds and organoleptic characteristics similar to the 'Montenegrina' tangerine. Embryos were isolated from seeds, cultivated in vitro, and acclimated in a greenhouse. Four random primers were used to identify 54 plants of sexual origin from a total of 202 individuals. The degree of polymorphism of each primer was reflected in the number of zygotic plants obtained per primer. Cluster analysis of parents and progeny separated the individuals into distinct groups with a maximum genetic dissimilarity of 20%.
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页码：123 / 127
页数：5
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