Rapid detection of Salmonella with Recombinase Aided Amplification

被引:77
作者
Zhang, Xiaoping [1 ]
Guo, Lichuan [2 ]
Ma, Ranran [2 ]
Cong, Lijuan [3 ]
Wu, Zhonghua [4 ]
Wei, Ying [1 ]
Xue, Shijie [1 ]
Zheng, Wei [4 ]
Tang, Saijun [5 ]
机构
[1] Zhejiang Entry Exit Inspect & Quarantine Bur, Hangzhou Airport Off, Hangzhou, Zhejiang, Peoples R China
[2] Jiangsu Qitian Biotech Co Ltd, Wuxi, Jiangsu, Peoples R China
[3] Airport Ind Zone B6, Beijing, Peoples R China
[4] Int Travel Healthcare Ctr, Hangzhou, Zhejiang, Peoples R China
[5] China Agr Univ, Coll Biol Sci, Beijing, Peoples R China
关键词
Salmonella; Recombinase Aided Amplification; Foodborne; Food safety; POLYMERASE-CHAIN-REACTION; MEDIATED ISOTHERMAL AMPLIFICATION; REAL-TIME PCR; FECAL SAMPLES; WATER SAMPLES; FOOD; OUTBREAK; STRAINS; CHICKEN; PRODUCE;
D O I
10.1016/j.mimet.2017.06.011
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Rapid Salmonella detection using Recombinase Aided Amplification was established. The reaction completes in 20 min at 39 degrees C and can be performed with a portable device. Once further improved, this method should be a great choice for monitoring contamination, such as foodborne Salmonella or for similar purposes.
引用
收藏
页码:202 / 204
页数:3
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