Rapid detection of Salmonella with Recombinase Aided Amplification

被引:77
作者
Zhang, Xiaoping [1 ]
Guo, Lichuan [2 ]
Ma, Ranran [2 ]
Cong, Lijuan [3 ]
Wu, Zhonghua [4 ]
Wei, Ying [1 ]
Xue, Shijie [1 ]
Zheng, Wei [4 ]
Tang, Saijun [5 ]
机构
[1] Zhejiang Entry Exit Inspect & Quarantine Bur, Hangzhou Airport Off, Hangzhou, Zhejiang, Peoples R China
[2] Jiangsu Qitian Biotech Co Ltd, Wuxi, Jiangsu, Peoples R China
[3] Airport Ind Zone B6, Beijing, Peoples R China
[4] Int Travel Healthcare Ctr, Hangzhou, Zhejiang, Peoples R China
[5] China Agr Univ, Coll Biol Sci, Beijing, Peoples R China
关键词
Salmonella; Recombinase Aided Amplification; Foodborne; Food safety; POLYMERASE-CHAIN-REACTION; MEDIATED ISOTHERMAL AMPLIFICATION; REAL-TIME PCR; FECAL SAMPLES; WATER SAMPLES; FOOD; OUTBREAK; STRAINS; CHICKEN; PRODUCE;
D O I
10.1016/j.mimet.2017.06.011
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Rapid Salmonella detection using Recombinase Aided Amplification was established. The reaction completes in 20 min at 39 degrees C and can be performed with a portable device. Once further improved, this method should be a great choice for monitoring contamination, such as foodborne Salmonella or for similar purposes.
引用
收藏
页码:202 / 204
页数:3
相关论文
共 31 条
[1]   Current concepts in the diagnosis and treatment of typhoid fever [J].
Bhutta, Zulfiqar A. .
BMJ-BRITISH MEDICAL JOURNAL, 2006, 333 (7558) :78-82B
[2]  
Bolton FJ, 2000, J AOAC INT, V83, P299
[3]   Salmonella, the host and disease:: a brief review [J].
Coburn, Bryan ;
Grassl, Guntram A. ;
Finlay, B. B. .
IMMUNOLOGY AND CELL BIOLOGY, 2007, 85 (02) :112-118
[4]   Real-time PCR detection of Salmonella in suspect foods from a gastroenteritis outbreak in Kerr County, Texas [J].
Daum, LT ;
Barnes, WJ ;
McAvin, JC ;
Neidert, MS ;
Cooper, LA ;
Huff, WB ;
Gaul, L ;
Riggins, WS ;
Morris, S ;
Salmen, A ;
Lohman, KL .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (08) :3050-3052
[5]   A simple, rapid, cost-effective and sensitive method for detection of Salmonella in environmental and pecan samples [J].
Dobhal, S. ;
Zhang, G. ;
Rohla, C. ;
Smith, M. W. ;
Ma, L. M. .
JOURNAL OF APPLIED MICROBIOLOGY, 2014, 117 (04) :1181-1190
[6]   Preharvest Salmonella Detection for Evaluation of Fresh Ground Poultry Product Contamination [J].
Evans, Nicholas P. ;
Evans, Robert D. ;
Regalado, Jason ;
Sullivan, Joseph F. ;
Dutta, Vikrant ;
Elvinger, Francois ;
Pierson, F. William .
JOURNAL OF FOOD PROTECTION, 2015, 78 (07) :1266-1271
[7]   Salmonella enterica Serovar Typhimurium Skills To Succeed in the Host: Virulence and Regulation [J].
Fabrega, Anna ;
Vila, Jordi .
CLINICAL MICROBIOLOGY REVIEWS, 2013, 26 (02) :308-341
[8]   Rapid and Sensitive Salmonella Typhi Detection in Blood and Fecal Samples Using Reverse Transcription Loop-Mediated Isothermal Amplification [J].
Fan, Fenxia ;
Yan, Meiying ;
Du, Pengcheng ;
Chen, Chen ;
Kan, Biao .
FOODBORNE PATHOGENS AND DISEASE, 2015, 12 (09) :778-786
[9]   Detection of Live Salmonella sp Cells in Produce by a TaqMan-Based Quantitative Reverse Transcriptase Real-Time PCR Targeting invA mRNA [J].
Gonzalez-Escalona, Narjol ;
Hammack, Thomas S. ;
Russell, Mindi ;
Jacobson, Andrew P. ;
De Jesus, Antonio J. ;
Brown, Eric W. ;
Lampel, Keith A. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (11) :3714-3720
[10]  
Hsieh K., 2012, Angewandte Chemie, V124, P4980, DOI DOI 10.1002/ANGE.201109115