Study on separative structure of EnFET to detect acetylcholine

In this study, our laboratory used a separative structure of extended gate field effect transistor (EGFET) to detect pH value and constructed an enzyme modified field effect transistor (EnFET) by coupling them with a membrane containing immobilized acetylcholinesterase (AcChE). The enzyme-catalyzed hydrolysis reaction of acetylcholine seemed to be resulted in the pH change of the membrane due to the formation of acetic acid. This structure has a lot of advantages, such as being insensitive toward light, being simple to in its passivation and package, and the flexibility of shape in the extended gate area, etc. In addition, it is easier to fabricate and package the sensors, and the measurement is simple for the application of disposable biosensor. (C) 2000 Elsevier Science B.V. All rights reserved.