Interaction of bovine serum albumin with self-assembled nanoparticles of 6-O-cholesterol modified chitosan

被引：50
作者：
Li, Xuemin [1 ,2 ]
Chen, Mingmao [1 ,2 ]
Yang, Wenzhi [1 ,2 ]
Zhou, Zhimin [1 ,2 ]
Liu, Lingrong [1 ,2 ]
Zhang, Qiqing [1 ,2 ,3 ]
机构：
[1] Chinese Acad Med Sci, Inst Biomed Engn, Tianjin 300192, Peoples R China
[2] Peking Union Med Coll, Key Lab Biomed Mat Tianjin, Tianjin 300192, Peoples R China
[3] Xiamen Univ, Biomed Engn Res Ctr, Technol Res Ctr Biomed Engn Xiamen City, Key Lab Biomed Engn Fujian Prov, Xiamen 361005, Peoples R China
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2012年 / 92卷
基金：
中国国家自然科学基金;
关键词：
6-O-Cholesterol modified chitosan; Bovine serum albumin; Nanoparticle; Interaction; Polymeric amphiphile; ISOTHERMAL TITRATION CALORIMETRY; CIRCULAR-DICHROISM SPECTROSCOPY; PROTEIN SECONDARY STRUCTURE; AGGREGATED NANOPARTICLES; THERMODYNAMICS; ADSORPTION; DELIVERY; COMPLEXATION; FLUORESCENCE; SEGMENTS;
D O I：
10.1016/j.colsurfb.2011.11.030
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In order to understand the nanomedicine and nanotoxicological effects of self-assembled nanoparticles of 6-O-cholesterol modified chitosan (O-CHCS NPs) as a carrier for drug delivery systems, the interaction between O-CHCS NPs and bovine serum albumin (BSA) was studied by spectroscopy and calorimetric methods. The morphology of the complex between O-CHCS NPs and BSA observed by transmission electron microscope (TEM) was almost spherical shape. The size and the zeta potential of the complex increased with the concentration of O-CHCS NPs increasing. The fluorescence spectroscopy indicated that the micro-environment around the tryptophan (Trp) residues in BSA had slight change due to only partially exposure of the Trp residues to water in the interaction process. Compared with free BSA, the addition of O-CHCS NPs led to the decrease of alpha-helical content of BSA and the increase of beta-strand content. Isothermal titration calorimeter (ITC) results showed that the binding reaction between O-CHCS NPs and BSA was exothermic and enthalpically driven. Therefore, it could be concluded that hydrogen bonding, hydrophobic and electrostatic interactions played a key role in the complex formation, and the formation mechanism was proposed accordingly. In addition, cytotoxicity assay implied that O-CHCS NPs were non-cytotoxic and biocompatible up to 200 mu g mL(-1). These data demonstrated the potential application of O-CHCS NPs for drug delivery. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：136 / 141
页数：6
相关论文
共 44 条
[1]   Preparation of nanoparticles by the self-organization of polymers consisting of hydrophobic and hydrophilic segments: Potential applications [J].
Akagi, Takami ;
Baba, Masanori ;
Akashi, Mitsuru .
POLYMER, 2007, 48 (23) :6729-6747
[2]   Self-assembly of polymer amphiphiles: Thermodynamics of complexation between bovine serum albumin and self-aggregate of cholesterol-bearing pullulan [J].
Akiyoshi, K ;
Nishikawa, T ;
Mitsui, Y ;
Miyata, T ;
Kodama, M ;
Sunamoto, J .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 1996, 112 (2-3) :91-95
[3]   Drug delivery systems: Entering the mainstream [J].
Allen, TM ;
Cullis, PR .
SCIENCE, 2004, 303 (5665) :1818-1822
[4]   Calorimetric and structural investigation of the interaction between bovine serum albumin and high molecular weight dextran in water [J].
Antonov, YA ;
Wolf, BA .
BIOMACROMOLECULES, 2005, 6 (06) :2980-2989
[5]   The protein-nanomaterial interface [J].
Asuri, Prashanth ;
Bale, Shyam Sundhar ;
Karajanagi, Sandeep S. ;
Kane, Ravi S. .
CURRENT OPINION IN BIOTECHNOLOGY, 2006, 17 (06) :562-568
[6]   Binding and fluorescence study on interaction of human serum albumin (HSA) with cetylpyridinium chloride (CPC) [J].
Bordbar, Abdol-Khalegh ;
Taheri-Kafrani, Asghar .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2007, 55 (01) :84-89
[7]   Physicochemical and Conformational Studies on BSA-Surfactant Interaction in Aqueous Medium [J].
Chakraborty, Tanushree ;
Chakraborty, Indranil ;
Moulik, Satya P. ;
Ghosh, Soumen .
LANGMUIR, 2009, 25 (05) :3062-3074
[8]   Preparation and characterization of self-assembled nanoparticles of 6-O-cholesterol-modified chitosan for drug delivery [J].
Chen, Mingmao ;
Liu, Yan ;
Yang, Wenzhi ;
Li, Xuemin ;
Liu, Lingrong ;
Zhou, Zhimin ;
Wang, Yinsong ;
Li, Ruifeng ;
Zhang, Qiqing .
CARBOHYDRATE POLYMERS, 2011, 84 (04) :1244-1251
[9]   The potential environmental impact of engineered nanomaterials [J].
Colvin, VL .
NATURE BIOTECHNOLOGY, 2003, 21 (10) :1166-1170
[10]   Interaction of bovine serum albumin with anionic surfactants [J].
Deep, S ;
Ahluwalia, JC .
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2001, 3 (20) :4583-4591
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