p21WAF1 is associated with CDK2 and CDK4 protein during HL-60 cell differentiation by TPA treatment

被引：6
|
作者：
Cho, JW
Jeong, YW
Kim, KS
Oh, JY
Park, JC
Lee, JC
Baek, WK
Suh, SI
Suh, MH
机构：
[1] Keimyung Univ, Sch Med, Dept Microbiol, Taegu 700712, South Korea
[2] Seonam Univ, Coll Med, Dept Microbiol, Namwon 590711, Chunpook, South Korea
来源：
CELL PROLIFERATION | 2001年 / 34卷 / 05期
关键词：
D O I：
10.1046/j.0960-7722.2001.00208.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
TPA-treated HL-60 cells are mainly arrested in G1 by p21(WAF1) accumulation. We investigate the downstream changes following such accumulation. Increased p21(WAF1) is associated with CDK2 and CDK4. pRb is dephosphorylated in the presence of p21-CDK2/4 complexes, and the Rb-E2F1 complex increases after TPA treatment, whereas the Rb-HDAC1 complex decreases slightly. Our results suggest that increased p21(WAF1) is associated with CDK2/4, and that these complexes induce pRb dephosphorylation. In turn, hypophosphorylated pRb are mainly complexed with E2F1, but HDAC1 appears not to be a key component in this process.
引用
收藏
页码：267 / 274
页数：8
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