Intravenous administration of human amniotic mesenchymal stem cells improves outcomes in rats with acute traumatic spinal cord injury.

被引：10
作者：
Zhou, Hong-Long [1 ]
Fang, Hua [1 ]
Luo, Hai-Tao [1 ]
Ye, Min-Hua [1 ]
Yu, Guo-Yong [2 ]
Zhang, Yan [1 ]
Mao, Guo-Hua [1 ]
Gao, Zi-Yun [1 ]
Cheng, Zu-Jue [1 ]
Zhu, Xin-Gen [1 ]
机构：
[1] Nanchang Univ, Dept Neurosurg, Affiliated Hosp 2, 1 Min De Rd, Nanchang 330006, Jiangxi, Peoples R China
[2] Peking Univ, Peoples Hosp, Dept Neurosurg, Beijing, Peoples R China
来源：
NEUROREPORT | 2020年 / 31卷 / 10期
基金：
中国国家自然科学基金;
关键词：
functional recovery; human amnion mesenchymal stem cell; intravenous transplantation; spinal cord injury; TRANSPLANTATION; RECOVERY; BARRIER;
D O I：
10.1097/WNR.0000000000001473
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We previously reported that intraspinal transplantation of human amniotic mesenchymal stem cells (hAMSCs) promotes functional recovery in a rat model of acute traumatic spinal cord injury (SCI). However, whether intravenous transplantation of hAMSCs also has therapeutic benefit remains uncertain. In this study, we assessed whether intravenous transplantation of hAMSCs improves outcomes in rats with acute traumatic SCI. In addition, the potential mechanisms underlying the possible benefits of this therapy were investigated. Adult female Sprague-Dawley rats were subjected to SCI using a weight drop device, and then hAMSCs or PBS were administered after 2 h via the tail vein. Our results indicated that transplanted hAMSCs could migrate to injured spinal cord lesion. Compared with the control group, hAMSCs transplantation significantly decreased the numbers of ED1(+)macrophages/microglia and caspase-3(+)cells, and reduced levels of inflammatory cytokines, such as tumor necrosis factor alpha, interleukin-6 and IL-1 beta. In addition, hAMSCs transplantation significantly attenuated Evans blue extravasation, promoted angiogenesis and axonal regeneration. hAMSCs transplantation also significantly improved functional recovery. These results suggest that intravenous administration of hAMSCs provides neuroprotective effects in rats after acute SCI, and could be an alternative therapeutic approach for the treatment of acute SCI.
引用
收藏
页码：730 / 736
页数：7
相关论文
共 23 条
[1]   Traumatic spinal cord injury [J].
Ahuja, Christopher S. ;
Wilson, Jefferson R. ;
Nori, Satoshi ;
Kotter, Mark R. N. ;
Druschel, Claudia ;
Curt, Armin ;
Fehlings, Michael G. .
NATURE REVIEWS DISEASE PRIMERS, 2017, 3
[2]   Traumatic Spinal Cord Injury-Repair and Regeneration [J].
Ahuja, Christopher S. ;
Nori, Satoshi ;
Tetreault, Lindsay ;
Wilson, Jefferson ;
Kwon, Brian ;
Harrop, James ;
Choi, David ;
Fehlings, Michael G. .
NEUROSURGERY, 2017, 80 (03) :S9-S22
[3]   Inflammogenesis of Secondary Spinal Cord Injury [J].
Anwar, M. Akhtar ;
Al Shehabi, Tuqa S. ;
Eid, Ali H. .
FRONTIERS IN CELLULAR NEUROSCIENCE, 2016, 10
[4]   Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion [J].
Baxter, MA ;
Wynn, RF ;
Jowitt, SN ;
Wraith, JE ;
Fairbairn, LJ ;
Bellantuono, I .
STEM CELLS, 2004, 22 (05) :675-682
[5]   Embryonic stem cells promote motor recovery and affect inflammatory cell infiltration in spinal cord injured mice [J].
Bottai, Daniele ;
Cigognini, Daniela ;
Madaschi, Laura ;
Adami, Raffaella ;
Nicora, Emanuela ;
Menarini, Mauro ;
Di Giulio, Anna Maria ;
Gorio, Alfredo .
EXPERIMENTAL NEUROLOGY, 2010, 223 (02) :452-463
[6]   Cell Transplantation for Spinal Cord Injury: Tumorigenicity of Induced Pluripotent Stem Cell-Derived Neural Stem/Progenitor Cells [J].
Deng, Junhao ;
Zhang, Yiling ;
Xie, Yong ;
Zhang, Licheng ;
Tang, Peifu .
STEM CELLS INTERNATIONAL, 2018, 2018
[7]   Intravenous multipotent adult progenitor cell treatment decreases inflammation leading to functional recovery following spinal cord injury [J].
DePaul, Marc A. ;
Palmer, Marc ;
Lang, Bradley T. ;
Cutrone, Rochelle ;
Tran, Amanda P. ;
Madalena, Kathryn M. ;
Bogaerts, Annelies ;
Hamilton, Jason A. ;
Deans, Robert J. ;
Mays, Robert W. ;
Busch, Sarah A. ;
Silver, Jerry .
SCIENTIFIC REPORTS, 2015, 5
[8]   Human amniotic membrane as an alternative source of stem cells for regenerative medicine [J].
Diaz-Prado, Silvia ;
Muinos-Lopez, Emma ;
Hermida-Gomez, Tamara ;
Cicione, Claudia ;
Esther Rendal-Vazquez, M. ;
Fuentes-Boquete, Isaac ;
de Toro, Francisco J. ;
Blanco, Francisco J. .
DIFFERENTIATION, 2011, 81 (03) :162-171
[9]   RISK OF TUMORIGENESIS IN FIRST-IN-HUMAN TRIALS OF EMBRYONIC STEM CELL NEURAL DERIVATIVES: ETHICS IN THE FACE OF LONG-TERM UNCERTAINTY [J].
Hess, Pascale G. .
ACCOUNTABILITY IN RESEARCH-POLICIES AND QUALITY ASSURANCE, 2009, 16 (04) :175-198
[10]   Global, regional, and national burden of traumatic brain injury and spinal cord injury, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016 [J].
James, Spencer L. ;
Theadom, Alice ;
Ellenbogen, Richard G. ;
Bannick, Marlena S. ;
Mountjoy-Venning, W. Cliff ;
Lucchesi, Lydia R. ;
Abbasi, Nooshin ;
Abdulkader, Rizwan ;
Abraha, Haftom Niguse ;
Adsuar, Jose C. ;
Afarideh, Mohsen ;
Agrawal, Sutapa ;
Ahmadi, Alireza ;
Ahmed, Muktar Beshir ;
Aichour, Amani Nidhal ;
Aichour, Ibtihel ;
Aichour, Miloud Taki Eddine ;
Akinyemi, Rufus Olusola ;
Akseer, Nadia ;
Alahdab, Fares ;
Alebel, Animut ;
Alghnam, Suliman A. ;
Ali, Beriwan Abdulqadir ;
Alsharif, Ubai ;
Altirkawi, Khalid ;
Andrei, Catalina Liliana ;
Anjomshoa, Mina ;
Ansari, Hossein ;
Ansha, Mustafa Geleto ;
Antonio, Carl Abelardo T. ;
Appiah, Seth Christopher Yaw ;
Ariani, Filippo ;
Asefa, Nigus Gebremedhin ;
Asgedom, Solomon Weldegebreal ;
Atique, Suleman ;
Awasthi, Ashish ;
Quintanilla, Beatriz Paulina Ayala ;
Ayuk, Tambe B. ;
Azzopardi, Peter S. ;
Badali, Hamid ;
Badawi, Alaa ;
Balalla, Shivanthi ;
Banstola, Amrit ;
Barker-Collo, Suzanne Lyn ;
Barnighausen, Till Winfried ;
Bedi, Neeraj ;
Behzadifar, Masoud ;
Behzadifar, Meysam ;
Bekele, Bayu Begashaw ;
Belachew, Abate Bekele .
LANCET NEUROLOGY, 2019, 18 (01) :56-87
123