FREQUENCY OF CELLS EXPRESSING CD44, A HEAD AND NECK CANCER STEM CELL MARKER: CORRELATION WITH TUMOR AGGRESSIVENESS

被引:123
作者
Joshua, Benzion [1 ]
Kaplan, Michael J. [1 ]
Doweck, Ilana [2 ]
Pai, Reetesh [3 ]
Weissman, Irving L. [4 ]
Prince, Mark E. [5 ]
Ailles, Laurie E. [6 ,7 ]
机构
[1] Stanford Univ, Med Ctr, Dept Otolaryngol Head & Neck Surg, Stanford, CA 94305 USA
[2] Carmel Hosp, Dept Otolaryngol Head & Neck Surg, Haifa, Israel
[3] Stanford Univ, Med Ctr, Dept Pathol, Stanford, CA 94305 USA
[4] Stanford Univ, Dept Dev Biol, Stanford Inst Stem Cell Biol & Regenerat Med, Sch Med, Stanford, CA 94305 USA
[5] Univ Michigan, Dept Otorhinolaryngol Head & Neck Surg, Ann Arbor, MI 48109 USA
[6] Univ Toronto, Ontario Canc Inst, Campbell Family Canc Res Inst, Toronto, ON, Canada
[7] Univ Toronto, Dept Med Biophys, Toronto, ON, Canada
来源
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2012年 / 34卷 / 01期
关键词
head and neck; squamous cell carcinoma; cancer; stem cells; CD44; ACUTE MYELOID-LEUKEMIA; UNITED-STATES; ALDEHYDE DEHYDROGENASE; ADHESION MOLECULES; PROGNOSTIC-FACTOR; TONGUE CANCER; CARCINOMA; SURVIVAL; IDENTIFICATION; PROGRESSION;
D O I
10.1002/hed.21699
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
Background. We previously identified by flow cytometry a Lineage-CD44+ (Lin-CD44+) subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma (HNSCC). We now correlate clinical and histologic factors with Lin-CD44+ cell frequency. Methods. The study included 31 patients with HNSCC, of whom 87% had stage IV disease. The frequency of Lin-CD44+ cells and the success of xenografting patient tumors in mice were correlated with clinical and pathologic data. Results. The mean frequency of Lin-CD44+ cells was 25% (0.4%-81%). It was 36% in patients who had recurrence versus 15% for those without recurrence (p = .04). Successful xenograft implantation occurred in 53%. Seventy-five percent of patients with successful xenografts had recurrence versus 21% of patients with unsuccessful xenografts (p = .003). Conclusions. Successful xenograft implantation and a high frequency of Lin-CD44+ cells correlate with known poor prognostic factors such as advanced T classification and recurrence. These findings may support the stem cell concept in HNSCC. (C) 2011 Wiley Periodicals, Inc. Head Neck 34: 42-49, 2012
引用
收藏
页码:42 / 49
页数:8
相关论文
共 39 条
  • [1] Ailles L, 2009, METHODS MOL BIOL, V568, P175, DOI 10.1007/978-1-59745-280-9_11
  • [2] Cancer stem cells in solid tumors
    Ailles, Laurie E.
    Weissman, Irving L.
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 2007, 18 (05) : 460 - 466
  • [3] Gains and losses of adhesion molecules (CD44, E-cadherin, and β-catenin) during oral carcinogenesis and tumour progression
    Bánkfalvi, A
    Krassort, M
    Buchwalow, IB
    Végh, A
    Felszeghy, E
    Piffkó, J
    [J]. JOURNAL OF PATHOLOGY, 2002, 198 (03) : 343 - 351
  • [4] Lack of expression of Thy-1 (CD90) on acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo
    Blair, A
    Hogge, DE
    Ailles, LE
    Lansdorp, PM
    Sutherland, HJ
    [J]. BLOOD, 1997, 89 (09) : 3104 - 3112
  • [5] Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell
    Bonnet, D
    Dick, JE
    [J]. NATURE MEDICINE, 1997, 3 (07) : 730 - 737
  • [6] CD44 as prognostic factor in oral and oropharyngeal squamous cell carcinoma
    Carinci, F
    Stabellini, G
    Calvitti, M
    Pelucchi, S
    Targa, L
    Farina, A
    Pezzetti, F
    Pastore, A
    [J]. JOURNAL OF CRANIOFACIAL SURGERY, 2002, 13 (01) : 85 - 89
  • [7] Aldehyde dehydrogenase 1 is a putative marker for cancer stem cells in head and neck squamous cancer
    Chen, Yu-Chih
    Chen, Yi-Wei
    Hsu, Han-Shui
    Tseng, Ling-Ming
    Huang, Pin-I
    Lu, Kai-Hsi
    Chen, Dow-Tien
    Tai, Lung-Kuo
    Yung, Ming-Chi
    Chang, Shih-Ching
    Ku, Hung-Hai
    Chiou, Shih-Hwa
    Lo, Wen-Liang
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 385 (03) : 307 - 313
  • [8] SINGLE-MARKER IDENTIFICATION OF HEAD AND NECK SQUAMOUS CELL CARCINOMA CANCER STEM CELLS WITH ALDEHYDE DEHYDROGENASE
    Clay, Matthew R.
    Tabor, Mark
    Owen, John Henry
    Carey, Thomas E.
    Bradford, Carol R.
    Wolf, Gregory T.
    Wicha, Max S.
    Prince, Mark E.
    [J]. HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, 2010, 32 (09): : 1195 - 1201
  • [9] Trends in palatine tonsillar cancer incidence and mortality rates in the United States
    Golas, Sylvia M.
    [J]. COMMUNITY DENTISTRY AND ORAL EPIDEMIOLOGY, 2007, 35 (02) : 98 - 108
  • [10] González-Moles MA, 2007, ONCOL REP, V18, P389