Free-Form-Fabricated Commercially Pure Ti and Ti6Al4V Porous Scaffolds Support the Growth of Human Embryonic Stem Cell-Derived Mesodermal Progenitors

被引：40

作者：

de Peppo, G. M. ^{[1
,2
]}

Palmquist, A. ^{[1
,2
]}

Borchardt, P. ^{[2
,3
]}

Lenneras, M. ^{[2
]}

Hyllner, J. ^{[2
,4
]}

Snis, A. ^{[5
]}

Lausmaa, J. ^{[2
,3
]}

Thomsen, P. ^{[1
,2
]}

Karlsson, C. ^{[1
,2
]}

机构：

[1] Gothenburg Univ, Sahlgrenska Acad, Dept Biomat, S-41346 Gothenburg, Sweden

[2] BIOMATCELL VINN Excellence Ctr Biomat & Cell Ther, S-41346 Gothenburg, Sweden

[3] SP Tech Res Inst Sweden, S-50115 Boras, Sweden

[4] Cellartis AB, Div Res & Dev, S-41346 Gothenburg, Sweden

[5] Arcam AB, Div Res & Dev, S-43137 Gothenburg, Sweden

来源：

SCIENTIFIC WORLD JOURNAL | 2012年

基金：

瑞典研究理事会;

关键词：

TITANIUM-ALLOY IMPLANTS; MARROW STROMAL CELLS; ALKALINE-PHOSPHATASE; BONE; CONSTRUCTS; METALS;

D O I：

10.1100/2012/646417

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Commercially-pure titanium (cp-Ti) and the titanium-aluminum-vanadium alloy (Ti6Al4V) are widely used as reconstructive implants for skeletal engineering applications, due to their good mechanical properties, biocompatibility and ability to integrate with the surrounding bone. Electron beam melting technology (EBM) allows the fabrication of customized implants with tailored mechanical properties and high potential in the clinical practice. In order to augment the interaction with the biological tissue, stem cells have recently been combined with metallic scaffolds for skeletal engineering applications. We previously demonstrated that human embryonic stem cell-derived mesodermal progenitors (hES-MPs) hold a great potential to provide a homogeneous and unlimited supply of cells for bone engineering applications. This study demonstrates the effect of EBM-fabricated cp-Ti and Ti6Al4V porous scaffolds on hES-MPs behavior, in terms of cell attachment, growth and osteogenic differentiation. Displaying different chemical composition but similar surface properties, EBM-fabricated cp-Ti and Ti6Al4V scaffolds supported cell attachment and growth, and did not seem to alter the expression of genes involved in osteogenic differentiation and affect the alkaline phosphatase activity. In conclusion, interfacing hES-MPs to EBM-fabricated scaffolds may represent an interesting strategy for design of third-generation biomaterials, with the potential to promote implant integration in clinical conditions characterized by poor bone quality.

引用

页数：14

共 58 条

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