Antimicrobial potential of the food-grade additive carvacrol against uropathogenic E. coli based on membrane depolarization, reactive oxygen species generation, and molecular docking analysis

被引：21

作者：

Khan, Imran ^{[1
,2
,3
]}

Bahuguna, Ashutosh ^{[2
]}

Shukla, Shruti ^{[4
]}

Aziz, Faisal ^{[3
]}

Chauhan, Anil Kumar ^{[2
]}

Ansari, Mohd Bismillah ^{[5
]}

Bajpai, Vivek K. ^{[6
]}

Huh, Yun Suk ^{[1
]}

Kang, Sun Chul ^{[2
]}

机构：

[1] Inha Univ, BSRC, Dept Biol Engn, 100 Inha Ro, Incheon 22212, South Korea

[2] Daegu Univ, Dept Biotechnol, Gyongsan 712714, Gyeongbuk, South Korea

[3] Univ Minnesota, Hormel Inst, Austin, MN 55912 USA

[4] NIFTEM, Dept Food Sci & Technol, Sonipat 131028, Haryana, India

[5] Saudi Basic Ind Corp SABIC, SABIC Technol & Innovat Ctr, Riyadh 11551, Saudi Arabia

[6] Dongguk Univ Seoul, Dept Energy & Mat Engn, 30 Pildong Ro 1 Gil, Seoul 04620, South Korea

来源：

MICROBIAL PATHOGENESIS | 2020年 / 142卷

基金：

新加坡国家研究基金会;

关键词：

Urinary tract infection; Carvacrol; Escherichia coli; Extended-spectrum beta-lactamase; Reactive oxygen species; LACTOBACILLUS-PLANTARUM; ESCHERICHIA-COLI; IN-VITRO; RESISTANCE;

D O I：

10.1016/j.micpath.2020.104046

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The antibiotic resistance of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli has increased drastically in recent years. In our study, we determined the principle mechanisms of action for the food-grade additive carvacrol against ESBL E. coli isolated from the blood of patients with a urinary tract infection. Carvacrol, which has a minimum inhibitory concentration of 150 mu g/ml and a minimum bactericidal concentration of 300 mu g/ml, reduced E. coli cell counts in a time-dependent manner. After treatment with carvacrol, the E. coli killing time was found to be 120 min. Fluorescent staining confirmed an increase in bacterial cell death, greater membrane depolarization, and an elevated oxidative burst in carvacrol-treated E. coli. Carvacrol also induced the release of cellular DNA, proteins, and potassium ions from bacterial cells and reduced both the number of E. coli in invasion assays against macrophages and the levels of the inflammatory proteins TNF-alpha and COX-2. In addition, carvacrol was found to inhibit beta-lactamase enzyme activity (in vitro), which was supported by in silico results. Moreover, carvacrol inhibited motility, and protected against bacterial invasion. Overall, the findings suggest that carvacrol has significant antimicrobial potential against ESBL E. coli.

引用

页数：9