Survival of pathogenic Mycobacterium abscessus subsp massiliense in Acanthamoeba castellanii

被引:7
作者
da Silva, Joas L. [1 ]
Nguyen, Jan [1 ]
Fennelly, Kevin P. [1 ]
Zelazny, Adrian M. [2 ]
Olivier, Kenneth N. [1 ]
机构
[1] NHLBI, Pulm Branch, Div Intramural Res, NIH, Bldg 10, Bethesda, MD 20892 USA
[2] NIH, Microbiol Serv, Dept Lab Med, Clin Ctr, Bldg 10, Bethesda, MD 20892 USA
基金
美国国家卫生研究院;
关键词
Acanthamoeba castellanii; Mycobacterium abscessus; Chronic lung infection; VIRULENCE; COCULTURE; POLYPHAGA; AMEBAS;
D O I
10.1016/j.resmic.2017.10.001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We used an amoeba model to study the intracellular growth and cytotoxicity of clinical strains of Mycobacterium abscessus subsp. massiliense (Mabsm) isolated from 2 patients (one with cystic fibrosis, the other one with idiopathic bronchiectasis) during the early (smooth colonies) and late stage (rough colonies) of chronic pulmonary infection. Acanthamoeba castellanii were infected with Mabsm (MOI 100) and samples collected every 24 h for 72 h. Results showed Mabsm is able to survive in trophozoites and persist in cysts for at least 7 days. Late Mabsm demonstrated higher cytotoxicity toward A. castellanii when compared to early strains. A. castellanii is a useful in vitro host model to study infection of Mabsm clinical isolates. Published by Elsevier Masson SAS on behalf of Institut Pasteur.
引用
收藏
页码:56 / 60
页数:5
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