Isolation, nucleotide sequence and expression of a cDNA encoding feline granulocyte colony-stimulating factor

被引:4
作者
Dunham, SP [1 ]
Onions, DE [1 ]
机构
[1] Univ Glasgow, Dept Vet Pathol, Retrovirus Res Lab, Glasgow G61 1QH, Lanark, Scotland
基金
英国惠康基金;
关键词
feline G-CSF; polymerase chain reaction; nucleotide sequence; cytokine; bacterial expression;
D O I
10.1006/cyto.2001.0910
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cDNA encoding feline granulocyte colony stimulating factor (fG-CSF) was cloned from alveolar macrophages using the reverse transcriptase-polymerase chain reaction. The cDNA is 949 bp in length and encodes a predicted mature protein of 174 amino acids. Recombinant fG-CSF was expressed as a glutathione S-transferase fusion and purified by affinity chromatography. Biological activity of the recombinant protein was demonstrated using the murine myeloblastic cell line GNFS-60, which showed an ED50 for fG-CSF of approximately 2 ng/ml. (C) 2001 Academic Press.
引用
收藏
页码:347 / 351
页数:5
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