Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates

被引：31

作者：

Torelli, Riccardo ^{[1
]}

Cacaci, Margherita ^{[1
]}

Papi, Massimiliano ^{[3
]}

Sterbini, Francesco Paroni ^{[1
]}

Martini, Cecilia ^{[1
]}

Posteraro, Brunella ^{[2
]}

Palmieri, Valentina ^{[3
,4
]}

De Spirito, Marco ^{[3
]}

Sanguinetti, Maurizio ^{[1
]}

Bugli, Francesca ^{[1
]}

机构：

[1] Univ Cattolica SC, Inst Microbiol, Lgo F Vito 1, I-00168 Rome, Italy

[2] Univ Cattolica SC, Inst Hyg, Lgo F Vito 1, I-00168 Rome, Italy

[3] Univ Cattolica SC, Inst Phys, Lgo F Vito 1, I-00168 Rome, Italy

[4] Natl Res Council ISC CNR, Inst Complex Syst, Via Taurini 19, I-00185 Rome, Italy

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2017年 / 158卷

关键词：

Biofilm; Vancomycin; Alginate lyase; Deoxyribonuclease I; E; faecalis; faecium; ALGINATE LYASE; IN-VITRO; ASPERGILLUS-FUMIGATUS; RESISTANCE; THERAPY; DNASE; VANCOMYCIN; BACTERIA;

D O I：

10.1016/j.colsurfb.2017.07.010

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：349 / 355

页数：7

共 45 条

[1]

Alipour M., 2009, J ANTIMICROB CHEMOTH

[2] Alginate lyase enhances antibiotic killing of mucoid Pseudomonas aeruginosa in biofilms [J].

Alkawash, MA ;

Soothill, JS ;

Schiller, NL .

APMIS, 2006, 114 (02) :131-138

[3] Enterococcus faecalis Produces Abundant Extracellular Structures Containing DNA in the Absence of Cell Lysis during Early Biofilm Formation [J].

Barnes, Aaron M. T. ;

Ballering, Katie S. ;

Leibman, Rachel S. ;

Wells, Carol L. ;

Dunny, Gary M. .

MBIO, 2012, 3 (04)

[4] EFFECTS OF ALGINASE ON THE NATURAL-HISTORY AND ANTIBIOTIC-THERAPY OF EXPERIMENTAL ENDOCARDITIS CAUSED BY MUCOID PSEUDOMONAS-AERUGINOSA [J].

BAYER, AS ;

PARK, S ;

RAMOS, MC ;

NAST, CC ;

EFTEKHAR, F ;

SCHILLER, NL .

INFECTION AND IMMUNITY, 1992, 60 (10) :3979-3985

[5] Exopolysaccharide microchannels direct bacterial motility and organize multicellular behavior [J].

Berleman, James E. ;

Zemla, Marcin ;

Remis, Jonathan P. ;

Liu, Hong ;

Davis, Annie E. ;

Worth, Alexandra N. ;

West, Zachary ;

Zhang, Angela ;

Park, Hanwool ;

Bosneaga, Elena ;

van Leer, Brandon ;

Tsai, Wenting ;

Zusman, David R. ;

Auer, Manfred .

ISME JOURNAL, 2016, 10 (11) :2620-2632

[6] In Vitro Effect of Clarithromycin and Alginate Lyase Against Helicobacter Pylori Biofilm [J].

Bugli, F. ;

Palmieri, V. ;

Torelli, R. ;

Papi, M. ;

De Spirito, M. ;

Cacaci, M. ;

Galgano, S. ;

Masucci, L. ;

Sterbini, F. Paroni ;

Vella, A. ;

Graffeo, R. ;

Posteraro, B. ;

Sanguinetti, M. .

BIOTECHNOLOGY PROGRESS, 2016, 32 (06) :1584-1591

[7] In Vitro Interaction between Alginate Lyase and Amphotericin B against Aspergillus fumigatus Biofilm Determined by Different Methods [J].

Bugli, Francesca ;

Posteraro, Brunella ;

Papi, Massimiliano ;

Torelli, Riccardo ;

Maiorana, Alessandro ;

Sterbini, Francesco Paroni ;

Posteraro, Patrizia ;

Sanguinetti, Maurizio ;

De Spirito, Marco .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (03) :1275-1282

[8] Force measurements with the atomic force microscope: Technique, interpretation and applications [J].

Butt, HJ ;

Cappella, B ;

Kappl, M .

SURFACE SCIENCE REPORTS, 2005, 59 (1-6) :1-152

[9] Recent advances in superhydrophobic surfaces and their relevance to biology and medicine [J].

Ciasca, G. ;

Papi, M. ;

Businaro, L. ;

Campi, G. ;

Ortolani, M. ;

Palmieri, V. ;

Cedola, A. ;

De Ninno, A. ;

Gerardino, A. ;

Maulucci, G. ;

De Spirito, M. .

BIOINSPIRATION & BIOMIMETICS, 2016, 11 (01)

[10] Mapping viscoelastic properties of healthy and pathological red blood cells at the nanoscale level [J].

Ciasca, G. ;

Papi, M. ;

Di Claudio, S. ;

Chiarpotto, M. ;

Palmieri, V. ;

Maulucci, G. ;

Nocca, G. ;

Rossi, C. ;

De Spirito, M. .

NANOSCALE, 2015, 7 (40) :17030-17037

← 1 2 3 4 5 →