Direct cytotoxicity evaluation of 63S bioactive glass and bone-derived hydroxyapatite particles using yeast model and human chondrocyte cells by microcalorimetry

被引：13

作者：

Doostmohammadi, A. ^{[1
]}

Monshi, A. ^{[1
]}

Fathi, M. H. ^{[1
]}

Karbasi, S. ^{[2
]}

Braissant, O. ^{[3
]}

Daniels, A. U. ^{[3
]}

机构：

[1] Isfahan Univ Technol, Dept Mat Engn, Biomat Grp, Esfahan 8415683111, Iran

[2] Isfahan Univ Med Sci, Med Phys & Biomed Engn Grp, Sch Med, Esfahan 8174673461, Iran

[3] Univ Basel, Lab Biomech & Biocalorimetry, Coalit Clin Morphol & Biomed Engn, Fac Med, Basel, Switzerland

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2011年 / 22卷 / 10期

关键词：

IN-VITRO; BIOCOMPATIBILITY; VIVO; OSTEOBLASTS;

D O I：

10.1007/s10856-011-4400-x

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this study, the cytotoxicity evaluation of prepared 63S bioactive glass and bone-derived hydroxyapatite particles with yeast and human chondrocyte cells was carried out using isothermal micro-nano calorimetry (IMNC), which is a new method for studying cell/biomaterial interactions. Bioactive glass particles were made via sol-gel method and hydroxyapatite was obtained from bovine bone. Elemental analysis was carried out by XRF and EDXRF. Amorphous structure of the glass and completely crystalline structure of HA were detected by XRD analysis. Finally, the cytotoxicity of bioactive glass and bone-derived HA particles with yeast and cultured human chondrocyte cells was evaluated using IMNC. The results confirmed the viability, growth and proliferation of human chondrocyte cells in contact with 63S bioactive glass, and bone-derived HA particles. Also the results indicated that yeast model which is much easier to handle, can be considered as a good proxy and can provide a rapid primary estimate of the ranges to be used in assays involving human cells. All of these results confirmed that IMNC is a convenient method which caters to measuring the cell-biomaterial interactions alongside the current methods.

引用

页码：2293 / 2300

页数：8

共 38 条

[1] CELLULAR-ACTIVITY OF OSTEOBLASTS IN THE PRESENCE OF HYDROXYAPATITE - AN INVITRO EXPERIMENT [J].