New method of derivatization and headspace solid-phase microextraction for gas chromatographic-mass spectrometric analysis of amphetamines in hair

被引：46

作者：

Liu, JT ^{[1
]}

Hara, K ^{[1
]}

Kashimura, S ^{[1
]}

Kashiwagi, M ^{[1
]}

Kageura, M ^{[1
]}

机构：

[1] Fukuoka Univ, Sch Med, Dept Forens Med, Jonan Ku, Fukuoka 8140180, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 758卷 / 01期

关键词：

derivatization; GC; amphetamines;

D O I：

10.1016/S0378-4347(01)00119-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method for hair analysis of methamphetamine (MAMP) and amphetamine (AMP) by gas chromatography-mass spectrometry (GC-MS) was developed using simultaneous headspace solid-phase microextraction (HS-SPME) with derivatization. After alkaline-digestion of hair, the analytes derivatized with heptafluoro-n-butyryl chloride were adsorbed on a polpdimethylsiloxane-coated fiber by HS-SPME and analyzed by GC-MS. Their mass spectra were, respectively, observable at 1 ng per sample. The standard curves in the range of 0.1-100 ng were linear. The intra-day coefficients of variation at each 0.5 ng were 12.5% for AMP and 3.8% for MAMP. The applicability of this method was demonstrated in some case studies. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：95 / 101

页数：7

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