Quantitative Sizing of Nano/Microparticles with a Tunable Elastomeric Pore Sensor

被引：228

作者：

Vogel, Robert ^{[1
]}

Willmott, Geoff ^{[2
]}

Kozak, Darby ^{[3
]}

Roberts, G. Seth ^{[3
]}

Anderson, Will ^{[3
]}

Groenewegen, Linda ^{[4
]}

Glossop, Ben ^{[4
]}

Barnett, Anne ^{[4
]}

Turner, Ali ^{[5
]}

Trau, Matt ^{[3
]}

机构：

[1] Univ Queensland, Sch Math & Phys, St Lucia, Qld 4072, Australia

[2] Ind Res Ltd, MacDiarmid Inst Adv Mat & Nanotechnol, Lower Hutt 5040, New Zealand

[3] Univ Queensland, Inst Bioengn & Nanotechnol, Biomarker Res & Dev Ctr, St Lucia, Qld 4072, Australia

[4] Izon Sci Ltd, Christchurch 8543, New Zealand

[5] Univ Oxford, Jenner Inst, Oxford OX3 7DQ, England

来源：

ANALYTICAL CHEMISTRY | 2011年 / 83卷 / 09期

关键词：

RESISTIVE-PULSE-TECHNIQUE; OPAL PHOTONIC CRYSTALS; SOLID-STATE NANOPORES; COULTER-TYPE COUNTERS; ELECTRON-MICROSCOPY; ANALYTICAL ULTRACENTRIFUGATION; SUBMICRON PARTICLES; SIZE DISTRIBUTION; LIGHT-SCATTERING; INSECT VIRUSES;

D O I：

10.1021/ac200195n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of a "size-tunable" polyurethane resistive pulse sensor for quantitative sizing of nano- and microparticles is presented. A linear relationship, as first suggested by Maxwell, between particle volume and change in electric resistance across the pore was observed. Particle sizes were quantified for a given size-tunable membrane, by first creating a linear calibration curve to a series of monodisperse carboxylated polystyrene particles of various diameters and then applying this curve to calculate the size of "unknown" nanoparticles. The diameters of a selection of synthetic and biological particles, being PMMA and nonfunctionalized polystyrene particles, along with biological nanoparticles (adenovirus) were calculated using this methodology. Calculated particle diameters and coefficients of variation were shown to be in good agreement with both transmission electron microscopy and dynamic light scattering results.

引用

页码：3499 / 3506

页数：8

共 55 条

[1]

Alberts B., 1994, MOL BIOL CELL

[2] Ultrafast laser fabrication of submicrometer pores in borosilicate glass [J].

An, Ran ;

Uram, Jeffrey D. ;

Yusko, Erik C. ;

Ke, Kevin ;

Mayer, Michael ;

Hunt, Alan J. .

OPTICS LETTERS, 2008, 33 (10) :1153-1155

[3]

BARR D, 1997, GENE THERAPY PROTOCO, P205

[4] OFF-AXIS RESPONSE FOR PARTICLES PASSING THROUGH LONG APERTURES IN COULTER-TYPE COUNTERS [J].

BERGE, LI ;

JOSSANG, T ;

FEDER, J .

MEASUREMENT SCIENCE AND TECHNOLOGY, 1990, 1 (06) :471-474

[5] Monitoring the homogeneity of adenovirus preparations (a gene therapy delivery system) using analytical ultracentrifugation [J].

Berkowitz, Steven A. ;

Philo, John S. .

ANALYTICAL BIOCHEMISTRY, 2007, 362 (01) :16-37

[6]

Bondoc LL, 1998, J IND MICROBIOL BIOT, V20, P317

[7] A resistive-pulse sensor chip for multianalyte immunoassays [J].

Carbonaro, A ;

Sohn, LL .

LAB ON A CHIP, 2005, 5 (10) :1155-1160

[8] ELECTROKINETIC MEASUREMENTS WITH SUBMICRON PARTICLES AND PORES BY RESISTIVE PULSE TECHNIQUE [J].

DEBLOIS, RW ;

BEAN, CP ;

WESLEY, RKA .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1977, 61 (02) :323-335

[9] COUNTING AND SIZING OF SUBMICRON PARTICLES BY RESISTIVE PULSE TECHNIQUE [J].

DEBLOIS, RW ;

BEAN, CP .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1970, 41 (07) :909-&

[10] COMPARATIVE MEASUREMENTS OF SIZE AND POLYDISPERSITY OF SEVERAL INSECT VIRUSES [J].

DEBLOIS, RW ;

UZGIRIS, EE ;

CLUXTON, DH ;

MAZZONE, HM .

ANALYTICAL BIOCHEMISTRY, 1978, 90 (01) :273-288

← 1 2 3 4 5 6 →